Abstract: Objective To compare and screen the therapeutic effects of Qihuang Yiqi Shexue Formula prepared by different processes on immune thrombocytopenia (ITP) model mice. Methods The Qihuang Yiqi Shexue Formula was extracted by three different processes: water extraction, water extraction and alcohol precipitation, water extraction alcohol extraction, and the test samples prepared by process 1, process 2, and process 3 were obtained respectively; 70 BALB/c mice were randomly divided into 7 groups: normal group, model group, Qihuang Yiqi Shexue Formula process 1, process 2, process 3, Weixuening granules group and prednisone group, with 10 mice in each group. The intraperitoneal injection of guinea pig anti-mouse platelet serum (APS) was used to establish an ITP mouse model; on the 8th day after the injection of APS, each group was given intragastric administration for 14 consecutive days. To observe and compare the effects of each drug group on the general condition of ITP model mice, peripheral blood platelet count (PLT), spleen and thymus index, and bone marrow megakaryocyte count. Results Compared with the normal group, the PLT count and Hb concentration in the peripheral blood of the model group were significantly decreased (P<0.05 or P<0.01), the spleen and thymus index were significantly increased (P<0.01), and the number of megakaryocytes in the bone marrow plate was significantly reduced (P<0.01); compared with the model group, the Qihuang Yiqi Shexue Formula prepared by the three processes can significantly increase the PLT count of ITP model mice ( P<0.01), and the PLT of the process group 1 and group 3 increased significantly, among which the process 3 groups are close to the normal group; in terms of improving the Hb level, there was an increase in the process 1, 2, and 3 groups, but only the process 3 group had a significant difference (P<0.05); in terms of reducing the organ index, the spleen and thymus indexes of the process 1 and 3 groups all decreased significantly (P<0.05 or P<0.01), but there was no statistical difference in the degree of reduction in the process 2 group (P>0.05); in terms of improving the number of bone marrow megakaryocytes, the number of bone marrow-producing megakaryocytes in the process 1, 2, and 3 groups all increased significantly (P<0.01), and the increase was the most obvious in the process 3 group, but there was no statistical difference in other indicators (P>0.05). The positive drugs Weixuening granules and the prednisone group have different degrees of improvement on the above indicators, while the process 3 group was better than Weixuening granules and prednisone in increasing the PLT count, reducing the spleen coefficient, and increasing the number of bone marrow megakaryocytes. Conclusion The Qihuang Yiqi Shexue Formula prepared by 3 different processes has a certain therapeutic effect on ITP model mice, and the therapeutic effect is process 3>process 1 >process 2.

Key words: immune thrombocytopenia (ITP), Qihuang Yiqi Shexue Formula, different extraction processes, comparison of curative effects, mice