Content determination and health risk assessment of pyrrolizidine alkaloid N-oxides in Vignae Semen

doi:10.19803/j.1672-8629.20220317

Abstract

Abstract: Objective To determine the contents of pyrrolizidine alkaloid N-oxides in Vignae Semen, and to assess the risks. Methods A UPLC-MS/MS method was established to determine the contents of monocrotaline N-oxide, echinatine N-oxide,intermedine N-oxide and lycosamine N-oxide. An Acquity UPLC BEH C18 (50 mm× 2.1 mm, 1.7 μm) was used as the chromatographic column. The mobile phase was eluted with acetonitrile-0.1% formic acid solution (10:90) at a constant flow rate of 0.35 mL·min^-1, and the injection volume was 2 μL. The electrospray spray ion source and positive ion multi-reaction monitoring mode were used. The contents were calculated using the standard curve method. Results The contents of monocrotaline N-oxide, echinatine N-oxide, intermedine N-oxide and lycosamine N-oxide in eight batches of Vignae Semen were 17.54-31.67 μg·kg^-1. The risk assessment showed that the health risk of Vignae Semen was low. Conclusion This study provides data for quality control and safety evaluation of pyrrolizidine alkaloids in Vignae Semen.

Key words: Vignae Semen, monocrotaline N-oxide, echinatine N-oxide, intermedine N-oxide, lycosamine N-oxide, UPLC-MS/MS, risk assessment

CLC Number:

R917

ZAN Ke, ZHOU Ying, LI Yaolei, WANG Ying, JIN Hongyu, ZUO Tiantian, MA Shuangcheng. Content determination and health risk assessment of pyrrolizidine alkaloid N-oxides in Vignae Semen[J]. Chinese Journal of Pharmacovigilance, 2022, 19(11): 1186-1190.

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