Acute toxicity of anti-gastric cancer active ingredients of Ferula ferulaeoides

doi:10.19803/j.1672-8629.20230136

Abstract

Abstract: Objective To study the acute toxicity and safety of anti-gastric cancer active ingredients of Ferula ferulaeoides so as to provide reference for the research and development of anti-gastric cancer drugs and clinical medications. Methods The mixed suspension of anti-gastric cancer active ingredients of Ferula ferulaeoides was administered intragastrically to mice at the maximum dose. The mice were weighed before and 14 days after administration. The health and death of mice were observed for 14 consecutive days. On the 14th day after administration, serum was collected, and the white blood cell count (WBC), red blood cell count (RBC), hemoglobin concentration (HGB), platelet count (PLT) and lymphocyte percentage (LYM) of mice were determined. The indexes of the liver, spleen and kidney were calculated by dissecting mice. The pathological sections of the liver, spleen, kidney and stomach were prepared to observe the pathological injury to the organs. Results The maximum dosage of anti-gastric cancer active ingredients of Ferula ferulaeoides for mice was 5.00 g·kg^-1. During administration, the mice in the group of anti-gastric cancer active ingredients of Ferula ferulaeoides appeared listless, tired, with erection and dark blood color of hair and sticky feces. Compared with blank control group, there was no significant difference in body weight before and 14 d after administration (P > 0.05), or in white blood cell count (WBC), red blood cell count (RBC), hemoglobin concentration (HGB), platelet count (PLT) and lymphocyte percentage (LYM) (P > 0.05), or in the indexes of the liver, spleen and kidney (P > 0.05). Histopathological results showed no significant pathological changes in the liver, spleen, kidney or stomach. Conclusion The anti-gastric cancer active ingredients of Ferula ferulaeoides cause no serious toxic reactions within 14 days, without any significant effect on the weight and diet of mice or on the liver, spleen, kidney and stomach of mice, but irritative reactions may occur within 2h of administration. This may be caused by nerve irritation or gastrointestinal irritation of the anti-gastric cancer active ingredients of Ferula ferulaeoides.

Key words: Ferula ferulaeoides, anti-gastric cancer active ingredients, mice, intragastrical administration, acute toxicity

CLC Number:

YANG Minghan, QIAO Meiling, LUO Jiaoyang, YANG Meihua, SHENG Ping. Acute toxicity of anti-gastric cancer active ingredients of Ferula ferulaeoides[J]. Chinese Journal of Pharmacovigilance, 2023, 20(8): 880-884.

References

[1] SHEN GM.Zhong Cao Yao Cong Shu: A Wei (中草药丛书: 阿魏)[M]. Urumqi: Xinjiang People's Publishing House, 1986.
[2] Xinjiang Institute of Biological Soil and Desert Research. Annals of Medicinal plants in Xinjiang (新疆药用植物志)[M]. Urumqi: Xinjiang People's Publishing House, 1977:106.
[3] SONG DW, ZHAO WJ, WU XP, et al.Study on chemical component of Ferula ferulaeoides[J]. Chinese Traditional and Herbal Drugs(中草药), 2006, 37(11): 1627-1629.
[4] SHENG P, TANG DP, MIAO LJ, et al.GC-MS fingerprints of essential oils of Ferula ferulaeoides from different habitats[J]. Chinese Journal of Modern Applied Pharmacy(中国现代应用药学), 2015, 32(1): 30-37.
[5] MENG H, LI G, HUANG J, et al.Sesquiterpenoid derivatives from Ferula ferulaeoides (Steud.)Korov[J]. Phytochemistry, 2013, 86(2): 151.
[6] ZHANG L, TONG XP, ZHANG J, et al.DAW22, a natural sesquiterpene coumarin isolated from Ferula ferulaeoides (Steud.) Korov. that induces C6 glioma cell apoptosis and endoplasmic reticulum (ER) stress[J]. Fitoterapia, 2015, 103: 46-54.
[7] WANG S.Studies on the fingerprint of active site of gastric cancer in vitro of Xinjiang Ferula ferulaeoides[D]. Urumqi: Xinjiang Medical University, 2015.
[8] YE BE, LIU F, XION YJ, et al.Effects of Anti-inflammatory and immunosuppressive on three species of Ferula[J]. Northwest Pharmaceutical Journal(西北药学杂志), 1993, 8(2): 72-75.
[9] GAO TT, YU FH, TAN Y, et al.Study on antimicrobial activity in vitro of extracts from three species of Ferula root[J]. Food Science and Technology(食品科技), 2013(24): 156-158.
[10] YANG MH, LUO JY, QIAO ML, et al.Mechanism of Uygur medicine root of Ferula ferulaeoides in resisting gastric cancer activity and inducing cell apoptosis and cell cycle arrest in vitro[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2018, 24(10): 112-122.
[11] QIAO ML, LUO JY, YANG MH, et al.Effect of Ferula ferulaeoides on growth and apoptosis of human gastric cancer MGC-803 transplantation tumor in nude mice[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2019, 44(13): 2827-2834.
[12] The research group of the project “Technical Guidelines for the study of acute toxicity of traditional Chinese medicine and natural drugs”. Technical guidelines for the study of acute toxicity of traditional Chinese medicine and natural drugs(中药、天然药物急性毒性研究技术指导原则)[S]. Beijing: Food and Drug Administration of the People's Republic of China, 2004.
[13] WANG R, LIU ZQ, WANG NN, et al.The study on acute toxicity test of anti-hypertensive new drug proline Danshensu borneol ester on mice[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2023, 20(7): 754-757.
[14] MO ZH, CHEN MX, WANG PC, et al.Acute toxicity of Huoxin Pill in mice[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2021, 18(10):924-929.
[15] CHEN QS, CAI SF, TAN YF, et al.Acute toxicity of mangrove plant Lumnitzera racemosa extract on mice[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2020, 17(2): 72-74, 86.
[16] XIONG YJ, LIU F, YE BE, et al.Effects of three species of Ferula on the gastroenteric tract[J]. Journal of Xinjiang Medical University(新疆医科大学学报), 1993(4): 300-302.