Abstract: Objective To investigate the pharmacological effects of Sanhan Huashi Granules on mouse pneumonia caused by human coronavirus 229E or OC43, and to provide guidance for clinical application. Methods The experiment was divided into 2 batches, and each batch of experimental animals were randomly divided into 7 groups according to body weight: normal group, viral infection model group, chloroquine phosphate tablets group and Lianhua Qingwen granules group, respectively. The Sanhan Huashi mice were divided into three groups: high dose group (11 g·kg^-1·d^-1, equivalent to 52.8 g crude drug g·kg^-1·d^-1), medium-dose group (5.5 g·kg^-1·d^-1, equivalent to 26.4 g crude drug g·kg^-1·d^-1) and low-dose group (2.75 g·kg^-1·d^-1, equivalent to 13.2 g crude drug g·kg^-1·d^-1). The mice model of pneumonia induced by human coronavirus 229E or OC43 was used to evaluate the pharmacological effect of Sanhan Huashi granules on mice with coronavirus pneumonia by detecting lung index and lung pathological changes. Results The high-dose, medium-dose and low-dose groups of Sanhan Huashi granules can reduce the lung index and alleviate the pathological injury of lung tissue in mice. Conclusion Sanhan huashi Granules have therapeutic effect on mice pneumonia caused by human coronavirus 229E or OC43 infection.

Key words: Sanhan Huashi Granules, Human coronavirus 229E(HCoV-229E), human coronavirus OC43(HCoV- OC43), mice, pneumonia, lung index, pathological changes