Mechanisms of Gegen Tang granules against viral pneumonia in mice based on immune regulation

doi:10.19803/j.1672-8629.20230612

Abstract

Abstract: Objective To study the efficacy and immunomodulatory effects of Gegen Tang granules on viral pneumonia induced by influenza A virus (IAV) in mice. Methods ICR mice, 13~15 g, were divided into the normal control group, model control group, oseltamivir phosphate positive control group and high-, medium- and low-dose Gegen Tang granule groups (6.6, 3.3 and 1.7 g^-1·kg^-1·d^-1), with 10 mice in each group. A viral pneumonia model of mice was established by IAV (FM1 strain) while relevant medication was given. Lung indexes and their inhibitory rate were observed. Levels of nucleic acid in lung tissue were detected by RT-PCR, while interleukin-6 (IL-6), interleukin-10 (IL-10) and tumor necrosis factor TNF-α in lung tissue were detected by ELISA. AV (FM1 strain) virus was used to infect mice with nasal drops, resulting in a death protection model. The death toll of mice within 2 weeks of infection was observed, and the mortality rate, death protection rate, average survival days and life extension rate of mice were calculated. Results The pulmonary index and viral load of lung tissue were significantly decreased (P<0.01), and the inhibitory rate of lung indexes was 50.73%. The content of lung inflammatory factor IL-10 in high and medium dose groups of Gegen Tang granules was significantly decreased (P<0.01), so was the content of lung inflammatory factor TNF-α in medium and low dose groups of Gegen Tang granules (P<0.01). The content of inflammatory factor IL-6 in lung tissue was significantly decreased in the three dose groups of Gegen Tang granules (P<0.01). The mortality rate of mice in the model group was 90%, and the average survival was 9.45 days, both of which were significantly decreased in the three dose groups of Gegen Tang granules, while the life extension rate was significantly increased (P<0.01). Conclusion Gegen Tang granules can improve the immune function of mice with viral pneumonia by adjusting the level of immune inflammatory factors while significantly reducing the lung index and viral load of the model mice so as to alleviate the pulmonary inflammatory damage to the model mice. They can help protect model mice from death.

Key words: influenza A virus, Gegen Tang granules, inflammatory factors, immune regulation, death protection, lung index, mice

CLC Number:

ZHAO Ronghua, SUN Jing, BAO Lei, GENG Zihan, TAO Xiali, ZHANG Jingsheng, PANG Bo, XU Yingli, CAO Shan, LI Shuran, GUO Shanshan, WANG Daohan, CUI Xiaolan. Mechanisms of Gegen Tang granules against viral pneumonia in mice based on immune regulation[J]. Chinese Journal of Pharmacovigilance, 2024, 21(2): 132-136.

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