Abstract: Objective To evaluate the pharmacological effects of Lingyang Ganmao Oral Liquid at the animal level, and to provide theoretical basis and data to support clinical precision medicine. Methods Six animal models were established according to the functional indications and clinical indications of Lingyang Ganmao Oral Liquid, the pneumonia model of mice infected by influenza virus FM1 strain and Streptococcus pneumoniae, the peritoneal capillary permeability model and body twisting model of mice induced by ice acetic acid, the cough model of mice induced by ammonia water, the trachea phenol red excretion model of mice. The antiviral, antibacterial, anti-inflammatory, analgesic, relieving cough and eliminating phlegm effects of Lingyang Ganmao Oral Liquid were evaluated by observing pulmonary index, inhibitory rate of lung index, OD value of Evanslan, writhing times, coughing times and phenol red excretion. Results Lingyang Ganmao Oral Liquid 20 mL·kg^-1·d^-1 can significantly inhibit the increase of lung index caused by influenza virus FM1, and significantly increase the secretion of phenol red in the tracheal of mice. The lung index and peritoneal capillary permeability of Streptococcus pneumoniae infected mice were increased. Lingyang Ganmao Oral Liquid 20, 15, 10 mL·kg^-1·d^-1 three dose groups had significant inhibitory effect on both of them, which could significantly reduce the frequency of cough caused by ammonia water in mice and prolong the incubation period of cough. Both 20 and 15 mL·kg^-1·d^-1 dose groups could significantly reduce the number of writhe caused by acetic acid in mice. Conclusion Lingyang Ganmao Oral Liquid of different doses has different degrees of action and multiple pharmacological effects, including antiviral, antibacterial, anti-inflammatory, analgesic, cough and expectorant.

Key words: Lingyang Ganmao Oral Liquid, antiviral, antibacterial, anti-inflammatory, anti-cough, remove phlegman, lung index, secretion of phenol red, mice