Abstract: Objective To observe the anti-fatigue effect of Ophiopogonin D by constructing a mouse model of exhaustive swimming exercise. Methods The mice were divided into three batches. The control group, swimming model group, low-dose (Ophiopogonin D 20 mg·kg^-1), medium-dose (Ophiopogonin D 40 mg·kg^-1) and high-dose administration groups (Ophiopogonin D 60 mg·kg^-1) were established. The weight-bearing swimming experiment was performed after a week of continuous intragastric administration. Serum creatine kinase, urea and triglyceride were measured via swimming test on the second day. One week after the second and third batches of mice were administered with the above dosages, the non-weight-bearing swimming experiment was conducted. The effects of high, medium and low doses of Ophiopogonin D on body weight, blood lactic acid and liver glycogen of mice were analyzed using the blood lactic acid detection kit, liver glycogen assay kit and otherwise. Results Compared with the swimming model group, the duration of weight-bearing swimming Ophiopogonin D mice was prolonged, the liver glycogen content was increased, the serum creatine kinase level was decreased, the serum triglyceride content was increased, and the area under the blood lactic acid curve was decreased after swimming. All the above indexes were of statistical significance (all P<0.05). Serum urea nitrogen decreased, but the change was not statistically significant (P>0.05). Conclusion Ophiopogonin D can significantly enhance exercise endurance, reduce lactic acid accumulation and increase glycogen reserve in mice.

Key words: ophiopogonin D, antifatigue, exhaustive swimming, blood lactic acid, hepatic glycogen, mice, intragastric administration