Toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish

doi:10.19803/j.1672-8629.20210529

Abstract

Abstract: Objective To study the toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish. Methods Zebrafish embryos were treated with multiple groups of samples while the wild type was used as the negative control. Embryonic development was observed at different time points. Results The four groups of Shujin Dingtong tablets showed obvious effects on the embryonic development of zebrafish at different concentrations. At a high concentration, embryonic toxicity was mainly manifested in developmental stagnation while at a medium concentration, developmental retardation and malformation of the main organs including the heart, brain and trunk were observed. At a low concentration, most of the embryos were close to normal, and a small number of embryos showed mild developmental delay. Conclusion The main manifestations of the effect of Shujin Dingtong tablets on zebrafish are embryonic development stagnation and lag at high and medium concentrations. Additionally, the toxicity of samples from the same manufacturer has a dose-dependent positive relationship with the amount of borax contained.

Key words: Shujin Dingtong tablet, zebrafish, embryonic development, acute toxicity, borax, stagnation, lag

CLC Number:

LIU Jing, WANG Feng, ZHANG Jingpu, MENG Jie, DAI Zhong, MA Shuangcheng. Toxic effect of Shujin Dingtong tablets on embryonic development of zebrafish[J]. Chinese Journal of Pharmacovigilance, 2023, 20(3): 262-265.

References

[1] NMPA. Drug Standard of Ministry of Public Health Traditional Chinese Medicine Preparations Volume 15 WS₃-B-3005-98[S]. 1998: 206.
[2] NMPA. State Food and Drug Administration National Drug Standard WS₃-B-3005-98-1[S]. 2005.
[3] YAN WW.Study on quality standard of Shujin Dingtong Tablets[J]. Chinese Traditional and Herbal Drugs(中草药), 2010, 41(7): 1113-1115.
[4] WANG SJ, ZHAO XJ.Determination of emodin and chrysphanol in Shujin Dingtong Tablet by RP-HPLC[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2009, 15(10): 34-35.
[5] CAI Q, LIU YQ.Determination of naringin in Shujin Dingtong Tablets by HPLC[J]. Chinese Traditional Patent Medicine(中成药), 2008, 30(4): 3-4.
[6] HUANG L, LIU CM, LUO SJ, et al.Research progress on hazard and detection methods of Borax[J]. Jiangxi Food Industry(江西食品工业), 2011 (2): 46-48.
[7] JIN X, ZHOU YL, CHEN XQ, et al.The effect of Borax on development of zebrafish[J]. Journal of Jining Medical University(济宁医学院学报), 2020, 43(4): 229-232.
[8] ZENG B, ZHANG CY, CHEN K.Application and research progress of model biological zebrafish in drug toxicity evaluation[J]. Natural Product Research and Development(天然产物研究与开发), 2020, 32: 1794-1801.
[9] LI HY, XIE Q, WANG C, et al.Research progress on application and mechanism of zebrafish in drug toxicology assessment[J].Chinese Traditional and Herbal Drugs(中草药), 2021, 52(1): 278-288.
[10] KATOCH S, PATIAL V.Zebrafish: an emerging model system to study liver diseases and related drug discovery[J]. Applied Toxicology, 2021, 41(1): 33-51.
[11] QUAN ZY, SUN ZX.Study on hepatotoxicity of aqueous extract of Polygoni multoflori radix and its main components based on Zebrfish[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2018, 15(1): 1-5.
[12] ZHANG KC, CAO YD, YAO F, et al.Acute toxicity of Euphorbiae pekinensis radix and vinegar-processing Euphorbiae pekinensis radix on zebrafish embryo[J]. Chinese Journal of Chinese Materia Medica(中国中药杂志), 2017, 42(12): 2291-2297.
[13] LIN QH, QU WJ, QIN HZ, et al.Acute toxicity study of different processed Arecae semen on zebrafish[J]. Asia-Pacific Traditional Medicine(亚太传统医药),2020, 16(2): 9-11.
[14] LIU J, ZHANG JP, MENG J, et al.Toxic effect of Niuhuang Zhenjing Pill on zebrafish embryo development[J].Drug Evaluation Research(药物评价研究),2017, 40(7): 931-933.
[15] LIU J, ZHANG JP, MENG J, et al.Acute toxic effect of Guci tablets on zebrafish embryo development[J].Drug Evaluation Research(药物评价研究),2018, 41(10): 1804-1809.
[16] TSHERING G, PLENGSURIYAKAM T, NA-BANGCHANG K, et al.Embryotoxicity evaluation of atractylodin and β-eudesmol using the zebrafish model[J]. Comparative Biochemistry and Physiology, Part C, 2021, 239(1),108869.
[17] LI Y, LIU XJ, SU SL, et al.Application progress and prospect of model organism zebrafish in activity screening and toxicity evaluation of traditional Chinese medicine[J]. Journal of Nanjing University of Traditional Chinese Medicine(南京中医药大学), 2020, 36(5): 715-720.