Archive

08 January 2016, Volume 13 Issue 1 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Influence of Host Factors CaMKⅡ and PACT in Activity Reduction of Influenza A Virus RNA Polymerase Compared with Ribavirin BAO Lei, GUO Shan-shan, CUI Xiao-lan 2016, 13(1): 1-4.  Abstract ( 402 )   PDF (1844KB) ( 201 )   Objective To observe the effect of host factors CaMK Ⅱ and PACT on influenza A virus RNA polymerase compared with ribavirin. Methods Dual luciferase reporter gene approach was used to detect relative expression of influenza A virus polymerase in 293T cells, judging the influence of influenza A virus polymerase by treatment with ribavirin and silencing protein CaMK Ⅱ and PACT. Results Silence CaMK Ⅱ and PACT all decreased the relative expression of influenza A virus RNA polymerase. But the inhibition was more obvious by dealing with ribavirin. Conclusion Ribavirin can control the expression of the influenza A virus RNA polymerase. The host factors CaMK Ⅱ.. and PACT play an important role in the process of synthesis of the influenza A virus RNA polymerase, and protein PACT is more significant than CaMK Ⅱ . References | Related Articles | Metrics

Select

Effects of Xigui on Learning and Memory Function in Mice DONG Shou-tang, WANG Cheng-jun, ZHANG Xu-qiang, ZHU Gui-rong, DU Yi-ming 2016, 13(1): 5-8.  Abstract ( 294 )   PDF (1012KB) ( 210 )   Objective To study the function of learning and memory of Xigui in mice. Methods The Methods of step-down test and step-through test were used to research the effects of Xigui on impairment of learning and memory in mice induced by scopomamine, sodium nitrite and ethanol. Results Compared with the model and solvent group, Xigui could significantly prolong the time of latency and decrease the number of errors on impairment of learning and memory in mice (P<0.05 orP<0.01). Conclusion Xigui could improve the learning and memory disorder of mice. References | Related Articles | Metrics

Select

Effects of Metformin on Ventricular Remodeling in Patients with Primary Hypertension and Type 2 Diabetes Mellitus JIN Xin-min 2016, 13(1): 9-12.  Abstract ( 350 )   PDF (1002KB) ( 215 )   Objective To explore the effects of metformin on ventricular remodeling in patients with primary hypertension and type 2 diabetes mellitus. Methods 162 patients with primary hypertension and type 2 diabetes mellitus treated in our hospital from May 2012 to April 2013 were selected. The patients were divided into test group (n=75) and control group (n=87) according to whether used metformin or not. Subgroup analyses of the patients in the test group were used with the time of taking metformin,the clinical and echocardiographic data of 162 patients were retrospectively analyzed to assess the effect of hypertension duration and duration of drug usage on ventricular remodeling in patients. Results After treatment, the LVMI, LVPWD and IVSD in the test group were significantly lower than those in the control group (P=0.002, 0.039, 0.008), metformin is an independent factor for LVMI and LVPWD (P=0.031, 0.043). The difference of LVMI level between taking metformin ≥12 months group and control group patients was statistically significant (P=0.038). Taking metformin time is the independent factor of LVMI (P=0.039), the LVMI of the patients with course of hypertension < 5 years and the IVSD、LVMI of the patients with course of hypertension ≥5 years in test group were significantly lower than the control group (P=0.032, 0.026, 0.023). Conclusion Metformin may attenuate left ventricular hypertrophy of patients with primary hypertension and type 2 diabetes mellitus, and then improve the ventricular remodeling. The improvement effect of the patients with longer course of hypertension and longer time of medication may be more significant. References | Related Articles | Metrics

Select

Study on Content of Volatile Oil in Leonurus japonicusHoutt. by Microwave-assisted Extraction SUN Ling, YANG Xiao, LI Shi-min, SUN Xiao-qian, SUN Rong 2016, 13(1): 13-15.  Abstract ( 288 )   PDF (1226KB) ( 113 )   Objective To discuss the extraction effect of volatile oil from Leonurus japonicus Houtt. by microwave-assisted extraction and optimum extract parameters. Methods Two Methods of hydro-distillation and microwave-assisted extraction were used for extracting volatile oil fromLeonurus japonicusHoutt., then optimized the extraction conditions: solvent volume, extracting power, extracting time, and gas chromatography was used for the qualitative identification and relative content analysis of component. Results The extracting yields of volatile oil from Leonurus japonicusHoutt. by microwave-assisted extraction of and hydro-distillation were 0.110% and 0.099%, respectively. Conclusion The extraction effect of volatile oil from Leonurus japonicusHoutt. by microwave-assisted extraction was better than hydro-distillation. References | Related Articles | Metrics

Select

Determination of Menthol and Borneol and HPLC Fingerprint in Cangerzi Rhinitis Drop Pills LIU Meng-ting, FANG Jing, TANG Li-ying, LIU Chong-de, Fu Mei-hong, WU Hong-wei, YANG Hong-jun 2016, 13(1): 16-19.  Abstract ( 335 )   PDF (1448KB) ( 162 )   Objective To establish a method of gas chromatography and the HPLC fingerprint to evaluate the quality of Cangerzi rhinitis drop pills. Methods Using the internal standard method to analyze the content of menthol and borneol in rhinitis dripping pills by gas chromatography. Samples were extracted using methanol and the methodology was established. Ethyl acetate was applied in extracting HPLC fingerprint sample. The detected wave length was 300 nm. The separation was performed with a gradient elution composed of methanol and 0.1% formic acid-water. Results The linear range of menthol was 0.312~0.015 6 mg·mL-1, r=0.999 9, the average recovery was 100.99% (RSD=3.97%, n=6). The linear range of borneol was 0.201 6~0.010 08 mg·mL-1, r=0.999 9, the average recovery rate was 97.61% (RSD=2.09%, n=6). The RSD of precision, repeatability and stability in two components were less than 3%. The concentration of menthol and borneol in Cangerzi rhinitis dropping pills was within the range of 1.34%~2.29% and 1.15%~1.61%, respectively. In the fingerprint, 19 characteristic peaks were established, and three of that were identified. Twenty batches of Cangerzi rhinitis drop pills were determined and the similarities of 18 batches were above 0.95. Conclusion Results show that the method of gas chromatography and fingerprint is convenient,accurate and reliable,which can be applied to control the quality of Cangerzi rhinitis dripping pills. References | Related Articles | Metrics

Select

Study on Drying and Molding Technology of Qianliean Concentrated Pills XIAO Yan-ying, WANG Hong-jun, ZHOU Ru-zhen, HUANG De-hong, LIU Min, WANG Yong 2016, 13(1): 20-23.  Abstract ( 285 )   PDF (1017KB) ( 212 )   Objective To optimize the best drying and molding technology of Qianliean concentrated pills. Methods Orthogonal test was employed for selecting the optimum drying technology of Qianliean concentrated pills by the content of salvianolic acid B and water content score, the factors to consider include the concentration ratio, drying temperature, drying time and vacuum degree. The molding technology was optimized with the molding rate and the time of disintegration score as index, and with the cutting speed, pushing time, concentration of ethanol and amount of alcohol as the factors. Results The best drying technology conditions were as follows: the concentration ratio was 1: 1.3, drying temperature was 65 ℃ , drying time was 60 min and vacuum degree was 0.08 MPa. Meanwhile, the factors of optimum molding technology were that when the concentration of ethanol was 80%, the cutting speed was 30 rpm, the pushing speed was 30 rpm and the amount of ethanol was 10%. Conclusion The optimized process is simple, feasible and stable, which can be used for the mass production of Qianliean concentrated pills. References | Related Articles | Metrics

Select

Progress and Implication of FDA Risk Communication in the United States TIAN Yi, YANG Yue, TIAN Li-juan 2016, 13(1): 24-27.  Abstract ( 378 )   PDF (997KB) ( 489 )   Objective To provide suggestions for drug risk communication in China by introducing the development and implementation effect of FDA risk communication system. Methods The material of FDA risk communication was summarized through the method of literature analysis and searching FDA official website. Results The development of FDA risk communication is more perfect, and through continuous exploration and practice FDA has formed a sound risk communication system and built up a perfect communication platform. Conclusion The drug risk communication is still weak in China. It is urgent to pay attention to Chinese risk communication and establish the sharing platform of risk communication. China should draw experiences from FDA to promote the development and improvement of Chinese drug risk communication from four aspects of focus, model, way and strategy. References | Related Articles | Metrics

Select

Surveillance for Therapeutic Inequivalence in Taiwan Area of China CHEN Zhi-dong 2016, 13(1): 28-31.  Abstract ( 304 )   PDF (1085KB) ( 306 )   Objective To share the experiences of surveillance for therapeutic inequivalence (TIE) in Taiwan area of China. Methods Based on the related data, TIE related issues such as definition, procedures for report and assessment, suspected reported cases, and active surveillance mechanism in Taiwan area of China were all introduced. Results There were 488 TIE reported cases from 2009 to 2014 in Taiwan area of China, only one case was found related to the drug of levothyroxine tablet after assessing all the TIE reported cases. Conclusion Now TIE surveillance is an important part of drug safety surveillance, which plays more and more roles in ensuring the safety of drug use as well as improving the quality of drug in Taiwan area of China. References | Related Articles | Metrics

Select

Advances in Establishment of Animal Models with Hepatic Cirrhosis and Thinking about Efficacy Evaluation of Traditional Chinese Medicine SHI Liang,SUN Rong 2016, 13(1): 32-35.  Abstract ( 317 )   PDF (1000KB) ( 162 )   Objective To introduce the research development on the advances in establishment of animal models with hepatic cirrhosis and think about efficacy evaluation of traditional Chinese medicine (TCM). Methods The related references in the last decades at home and abroad about cirrhosis model were collated, analyzed and summarized. Results At present, single factor induced modeling and composite factors induced modeling were mainly two categories of the modeling scheme. Conclusion We should choose suitable animal model on the basis of TCM’s characteristic and combine the research of cirrhosis model with clinical disease closely, in order to screen and evaluate candidate drug, and offer methodological ensurance for TCM’s pharmacological preclinical research and efficacy evaluation at the same time. References | Related Articles | Metrics

Select

Progress of Clinical Application and Safety of Tetanus Antitoxin YU Chao, XU Yu-ming, XU Jin, WAN Kai-hua, YUAN Xing-dong, LI Sui, ZHOU Juan 2016, 13(1): 36-41.  Abstract ( 328 )   PDF (1047KB) ( 508 )   Objective To understand the progress of the clinical utilization status and safety of tetanus antitoxin (TAT), and to provide references for post-marketing safety evaluation. Methods Domestic and foreign literatures on the clinical application and safety of TAT were reviewed for further analysis and comparison. Results Now TAT has been mainly used for passive immunity and tetanus therapeutics in clinic. Intensive safety studies showed that higher false-positive rate of its skin test mainly related to patient’s physique, medicine factor, infusion operation factor, and so on. Its main adverse effects included anaphylactic shock, erum sickness, and other kinds of adverse reactions. Conclusion TAT is still the first choice against tetanus of trauma patients in China, the paper suggests that medical workers must put emphasis on anaphylaxis and close observation, along with our country should expeditiously enact a specific against tetanus guidelines and standards of Chinese style, and promoting rational drug use. References | Related Articles | Metrics

Select

Reflection on Drug Regulatory Globalization Trend from the Heparin Sodium Event TIAN De-long, HUANG Zhi-lu 2016, 13(1): 42-46.  Abstract ( 309 )   PDF (1022KB) ( 414 )   Objective To provide some suggestions to cope with the challenges of drug regulatory globalization. Methods The investigation to the reason of event inspection, communication, rehabilitation and the deep risk of supply chain were analyzed through reviewing measures that FDA and relevant parties had given to heparin sodium event. Results Drug regulatory globalization is essentially the globalization of raw materials, intermediate products and preparations supply chain. Conclusion FDA’s global drug regulation has provided referential experience to China, and China should comply with the trend of regulatory globalization and take active countermeasures to ensure the safety of the drug supply chain. References | Related Articles | Metrics

Select

Analysis of the Side Effects of Contraceptives in the First-diagnosed Registration and Follow-up System in Jiangsu Province LIN Jie, SHI Wen-Hui, SUN Zhi-ming, BA Lei, ZHOU Jian, XU Hao-qin, CHEN Ying, YAO Jie 2016, 13(1): 47-59.  Abstract ( 343 )   PDF (1056KB) ( 165 )   Objective To evaluate the effect of the first-diagnosed registration and follow-up system for contraceptives of JiangsuProvince. Methods Women of reproductive health who needed taking contraceptives in family planning service institutions of the township or the county of 10 counties of Jiangsu province experienced health screening and follow-up registration from Aug. 1st, 2013 to Mar. 31st, 2015. Results Six different kinds of contraceptives were used among 40 women of childbearing age. The proportion of the first-time contraceptive users was 85.00%,and the high-risk population in contraceptive users was 91.20%, being lower than those of second-time contraceptive users, with no significant difference (P >0.05). All of 40 women finished the firsttime follow-up, with the percent of breast and gynecological examinations being 82.30% and 64.60% respectively. The prevalence of side effects due to contraceptives was 15.19%. Conclusion The result shows that the first-diagnosed registration and followup system for contraceptives could be implemented effectively. It should be strengthened to follow-up the women of childbearing age, especially those high-risk population so as to select the proper and high-effect contraceptives to enhance the science, pertinence and effectiveness of family planning and reproductive services. References | Related Articles | Metrics

Select

Clinical Efficacy and Pharmacoeconomics Evaluation of Extended-infusion of Biapenem in Treatment of Severe Pneumonia LI Lan, LI Ming-yan, LAI Jun-hua 2016, 13(1): 50-51.  Abstract ( 303 )   PDF (1026KB) ( 506 )   Objective To study the efficacy and economic benefit of extended-infusion of biapenem in treatment of severe pneumonia. Methods 58 patients were divided into 3 h extended-infusion group (EI) and 0.5 h intermit-infusion group (TI), given biapenem 0.3 g, q12 h~q6 h, respectively.The clinical effect was compared, and the pharmacoeconomics was analyzed by the least cost analysis. Results The effective rate of EI group was 63.33%, and the average cost was ( 4 057±296) yuan. The effective rate of TI group was 60.71%, the average cost was (5 624±308 ) yuan. The cost of EI group was significantly lower than that of the TI group (P <0.05). Conclusion It has prominent superiority for extended-infusion of biapenem to treat severe pneumonia, deserving of popularization and application. References | Related Articles | Metrics

Select

Analysis of Typical Irrational Medication Cases of Using Injective Proton Pump Inhibitors ZHOU Jian, SI Ji-gang 2016, 13(1): 52-54.  Abstract ( 453 )   PDF (976KB) ( 429 )   Objective To investigate and analyze the medication rationality of injective proton pump inhibitors (PPIs) for inpatients in a third class general hospital,so as to promote rational application of injective PPIs. Methods A random 200 medical records of using injective PPIs in hospitalized patients in 2014 were statistical analyzed, including patients’gender, age, department, indication, drug variety, dosage, course of treatment, and commented irrational medication examples. Results Injective PPIs are widely used in clinic, there is a certain of unreasonable medication in parts of medical records, including drug unreasonable selection, no indication of drug use and longer courses, etc. Conclusion Injective PPIs medication standards should be formulated by hospital, and the use of injective PPIs should be strictly controlled in clinic, avoiding excessive and irrational use of PPIs, reducing the cost of drugs and adverse drug reactions. References | Related Articles | Metrics