Influence of Host Factors CaMKⅡ and PACT in Activity Reduction of Influenza A Virus RNA Polymerase Compared with Ribavirin

Abstract

Abstract: Objective To observe the effect of host factors CaMK Ⅱ and PACT on influenza A virus RNA polymerase compared with ribavirin. Methods Dual luciferase reporter gene approach was used to detect relative expression of influenza A virus polymerase in 293T cells, judging the influence of influenza A virus polymerase by treatment with ribavirin and silencing protein CaMK Ⅱ and PACT. Results Silence CaMK Ⅱ and PACT all decreased the relative expression of influenza A virus RNA polymerase. But the inhibition was more obvious by dealing with ribavirin. Conclusion Ribavirin can control the expression of the influenza A virus RNA polymerase. The host factors CaMK Ⅱ.. and PACT play an important role in the process of synthesis of the influenza A virus RNA polymerase, and protein PACT is more significant than CaMK Ⅱ .

Key words: ribavirin, host factors PACT, CaMK Ⅱ , influenza A virus RNA polymerase

CLC Number:

R978.7

BAO Lei, GUO Shan-shan, CUI Xiao-lan. Influence of Host Factors CaMKⅡ and PACT in Activity Reduction of Influenza A Virus RNA Polymerase Compared with Ribavirin [J]. Chinese Journal of Pharmacovigilance, 2016, 13(1): 1-4.

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