Potential mechanism of Ginkgo biloba in the treatment of Alzheimer's disease based on network pharmacology

doi:10.19803/j.1672-8629.2022.09.08

Abstract

Abstract: Objective To explore the potential mechanism of Ginkgo biloba against Alzheimer’s disease (AD) using the network pharmacology method. Methods Via the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP), highly active chemical components of Ginkgo biloba were screened to predict potential targets. GeneCards, OMIM, CTD, TTD and Drugbank databases were searched for AD-related targets. Based on the Uniprot database, the potential targets of the active ingredients were converted while AD-related targets were crossed to obtain the AD-related targets of Ginkgo biloba. The STRING11.0 database was used to construct the target protein interaction network diagram. The key genes were screened, and the “Clue GO” plug-in in the software Cytoscape 3.8.2 was used for GO function enrichment analysis and KEGG signal pathway analysis of AD-related targets. Results A total of 32 effective chemical constituents of Ginkgo biloba leaves, 144 effective constituent targets, 470 AD-related targets, and 51 potential targets of Ginkgo biloba against AD were detected. Such active ingredients as β-sitosterol, stigmasterol and dibutyl phthalate mainly acted on such key targets as ALB, PTGS2, ESR1and BCL-2 by exerting anti-inflammatory, anti-oxidation and anti-apoptosis effects or by activating blood through the GABA synaptic signaling pathway, calcium signaling pathway or PI3K-Akt signaling pathway. Conclusion Based on the multi-component, multi-target and multi-pathway properties of traditional Chinese medicine, the potential mechanism of Ginkgo biloba against Alzheimer’s disease has been explored, which is expected to facilitate research on clinical application of Ginkgo biloba in the treatment of Alzheimer’s disease.

Key words: Ginkgo biloba, Alzheimer's disease, network pharmacology, β-sitosterol, stigmasterol, dibutyl phthalate

CLC Number:

R965.1

LI Yuanyuan, LIU Shengwei, REN Zhongyang, DING Ling, LIU Jie, LIAO Yuan. Potential mechanism of Ginkgo biloba in the treatment of Alzheimer's disease based on network pharmacology[J]. Chinese Journal of Pharmacovigilance, 2022, 19(9): 967-972.

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