Abstract: Objective To explore the biological mechanism by which Ginkgo Biloba protects against angina pectoris（AP）using network pharmacology. Methods The chemical active ingredients of Ginkgo Biloba leaves were screened from the Traditional Chinese Medicine System Pharmacology Analysis Platform (TCMSP) and the targets were collected. Such databases as TTD, TTTD, Drugbank, OMIM and GeneCards were searched for AP-related targets. A component-target-disease network was constructed with Cytoscape software. The protein-protein interaction (PPI) network was analyzed using STRING database. GO enrichment analysis and KEGG signal pathway analysis were performed on related targets. Results After selection, 30 active components of Ginkgo Biloba leaves and 111 potential target genes for AP were obtained. The active ingredients, such as quercetin, kaanide and ferulic acid, mainly acted on PIK3CG, MAPK14, NOS3, Akt1 and other key targets. Through fluid shear stress, PI3K-Akt, MAPK, calcium signaling pathway and other signaling pathways, these active ingredients protected against angina pectoris by means of anti-inflammation, anti-oxidation, anti-apoptosis, and regulation of ion channels. Conclusion The potential mechanism of Ginkgo Biloba for AP has been explored based on the multi-component, multi-target, and multi-pathway characteristics of traditional Chinese medicine, which can facilitate subsequent research and clinical application of Ginkgo Biloba in the treatment of AP and extended indications.

Key words: Ginkgo Biloba, angina pectoris, network pharmacology, protein-protein interaction, enrichment analysis