Network pharmacology of dendrobium alkaloids against cerebral ischemia-reperfusion injury

doi:10.19803/j.1672-8629.2022.03.05

Abstract

Abstract: Objective To study the mechanism by which dendrobium alkaloids combats cerebral ischemia-reperfusion injury based on network pharmacology. Methods The databases Pharmamapper and BATMAN-TCM were used to predict dendrobium alkaloids targets, and disease targets associated with cerebral ischemia-reperfusion injury (CIRI) were acquired via the GeneCards database. VENNY2.1.0 was used to map the potential targets of dendrobium alkaloids acting on CIRI before the potential targets were imported to Cytoscape3.8.2 software so as to construct a PPI network. Finally, the R package clusterProfiler was used for GO enrichment analysis and KEGG pathway analysis. Results A total of 214 dendrobine-related targets and 513 cerebral ischemia-reperfusion injury-related targets were found, 58 of which were intersection targets. After PPI analysis, the top ten core target genes were ALB, MAPK1, MAPK8, CASP3, IGF1, SRC, NOS3, MMP2, HSP90AA1 and ESR1. Based on the analysis of KEGG enrichment pathways, 14 significant pathways were obtained, including the Rap1 signaling pathway, MAPK signaling pathway and chemokine signaling pathway. Conclusion Dendrobium alkaloids can treat CIRI through multiple targets and multiple pathways, and may play a role in resisting cerebral ischemia-reperfusion injury mainly by means of inflammation and apoptosis.

Key words: dendrobium alkaloids, cerebral ischemia-reperfusion injury, network pharmacology

CLC Number:

R966R285

LIU Xiaoli, LIU Shengwei, LIAO Yuan, ZENG Fanping, LIU Jie. Network pharmacology of dendrobium alkaloids against cerebral ischemia-reperfusion injury[J]. Chinese Journal of Pharmacovigilance, 2022, 19(3): 252-258.

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