Mechanisms of Laportea bulbifera against rheumatoid arthritis

doi:10.19803/j.1672-8629.20210654

Abstract

Abstract: Objective To study the mechanism of the components of Laportea bulbifera absorbed in blood in the treatment of rheumatoid arthritis (RA) based on network pharmacology and molecular docking. Methods The protein targets of the components and disease-related protein targets were predicted in the database. The protein-protein interactions of the common protein targets were analyzed via the String database. The Metascape database was used to carry out Gene Ontology (GO) and pathways analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG). Network diagrams of “absorbed components - targets” and “components - targets - signal pathways” were constructed to obtain the key targets. Molecular docking between the components absorbed in blood and potential key targets were analyzed using AutoDock vina software to screen and verify the main active components and targets of Laportea bulbifera in the treatment of RA. Results The results showed that there were 22 components or metabolites in blood from Laportea bulbifera, which acted on 292 targets linked to RA. The core targets might be INS, IL-6, MAPK3 and TNF. The predicted target genes of Laportea bulbifera on RA mainly involved the response to cellular nitrogen compounds, hormone stimulation and organic nitrogen compound reaction. These components in blood from Laportea bulbifera might mainly act on TNF signaling pathway and Th17 cell differentiation signaling pathways. Molecular docking results showed that flavonoids and phenolic acids were the main components that could interact strongly with the core targets, and that they could have strong affinity with multiple targets. Conclusion The mechanism of Laportea bulbifera against RA is possibly associated with multi-ingredients, multi-targets and multi-pathways. The main active components (flavonoids and phenolic acids) act on key proteins including MAPK3, PTGS2, TNF and IL-6 and regulate Th17 cell differentiation and TNF signaling pathways.

Key words: Laportea bulbifera (Sieb.et Zucc.)Wedd., quercitrin, cynaroside, rheumatoid arthritis, network pharmacology, molecular docking

CLC Number:

R991
R994.11

FU Changli, LU Yuan, CHEN Siying, WANG Yonglin, LI Yongjun, WANG Aimin, LIU Chunhua. Mechanisms of Laportea bulbifera against rheumatoid arthritis[J]. Chinese Journal of Pharmacovigilance, 2023, 20(2): 171-176.

References

[1] HAN HY, SHEN JH.Research progress on the pathogenesis of rheumatoid arthritis[J]. World Latest Medicine Information(世界最新医学信息文摘), 2019, 19(93): 66-67, 69.
[2] WANG J, ZHAO QJ, ZHUO XB, et al.Research progress on therapeutic drugs for rheumatoid arthriti[J]. Journal of Pharmaceutical Practice(药学实践杂志), 2019, 37(6): 485-490.
[3] DING WC, HAN LY, QIAN K, et al.Mechanism of caulis sinomenii on rheumatoid arthritis based on network pharmacology[J]. China Medical Herald(中国医药导报), 2020, 17(17): 15-20.
[4] MA SZ.Research progress of traditional Chinese medicine in rheumatoid arthritis[J]. Shanxi Journal of Traditional Chinese Medicine(山西中医), 2020, 36(5): 58-60.
[5] CHEN YR, XU WF, MA ML, et al.Research progress of laportea bulbifera[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2019, 25(18): 214-220.
[6] WANG JUN, LU JINGLI, LAN YAN, et al.Total coumarins from Urtica dentata Hand prevent murine autoimmune diabetes via suppression of the TLR4-signaling pathways[J]. Journal of Ethnopharmacology, 2013, 146(1): 379-392.
[7] LU CL.Study on the effect of total coumarin from kenaf on dextran sodium sulfate-induced colitis in mice[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2012, 37(21): 3316-3320.
[8] LI Y, KANG NF, GONG ZP, et al.Intestinal absorption difference of Laportea bulbifera extract in rats with rheumatoid arthritis and normal rats by in situ intestinal circulating perfusion model[J]. Natural Product Research and Development(天然产物研究与开发), 2019, 31(11): 1896-1906.
[9] HAN HY.Preliminary study on the basis of national drug urtica dentata handand anti-inflammatory substance and metabolism[D]. Beijing: Beijing University of Chinese Medicine(北京中医药大学), 2018.
[10] CHEN SY, TANG J, WU D, et al.Simultaneous determination of five components of laportea bulbifera extract in R at plasma by LC-MS/MS: application to a pharmacokinetic study[J]. Revista Brasileira de Farmacognosia, 2020, 30: 1-8.
[11] XU YL, GUO SS, CUI XL, et al.Material basis and mechanism of Xiao'er Resuqing Oral Liquid on hand, foot and mouth disease based on network pharmacology and molecular docking[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2020, 11(25): 1-10.
[12] LUO TT, LU Y, YAN SK, et al.Network pharmacology in research of Chinese medicine formula: methodology, application and prospective[J]. Chin J Integr Med, 2020, 26(1): 72-80.
[13] ZHOU SH.Preliminary study on the relationship between mass control and antioxidation and anti-inflammatory activity of urtica dentata handand anti-inflammatory activity[D]. Guiyang: Guizhou University of Traditional Chinese Medicine (贵州中医药大学), 2017.
[14] LUO X.Study on the Mechanism of survey and induced immune tolerance mechanism of Hongmasu[D]. Wuhan: Huazhong University of Science and Technology (华中科技大学), 2010.
[15] SU ZQ, ZHAO ZY, XIE SN, et al.Effects of analgesia, anti-inflammation and immunosuppression of acetic ether extract of Chinese medicine Honghuoma[J]. Chinese Pharmacological Bulletin(中国药理学通报), 2009, 25(4): 559-560.