中国药物警戒 ›› 2023, Vol. 20 ›› Issue (3): 248-252.
DOI: 10.19803/j.1672-8629.20220371

• 基于感染共性机制的中药防治感染性疾病研究专栏 • 上一篇    下一篇

金振口服液对人冠状病毒肺炎热毒疫幼龄小鼠模型的干预机制

赵荣华1, 孙静1, 包蕾1, 耿子涵1, 李贵平2,3, 王道涵4, 李舒冉1, 张敬升1, 庞博1, 徐英莉1, 周利润1, 鲍岩岩1, 陈梦苹1, 郭姗姗1#, 崔晓兰1,*   

  1. 1中国中医科学院中药研究所生物安全实验室,北京 100700;
    2江苏康缘药业股份有限公司,江苏 连云港 222001;
    3中药制药过程新技术国家重点实验室,江苏 连云港 222001;
    4北京中医药大学东方医院儿科,北京 100078
  • 收稿日期:2022-07-01 出版日期:2023-03-15 发布日期:2023-03-17
  • 通讯作者: * 崔晓兰,女,博士,研究员,中药防治感染性疾病机制研究。E-mail：cuixiaolan2812@126.com; #为共同通信作者。
  • 作者简介:赵荣华,女,博士,副研究员·硕导,中药防治病毒性疾病药效机制研究。
  • 基金资助:
    国家重点研发计划（2021YFC1712903）; 中国中医科学院创新工程重大攻关项目资金资助（CI2021A04606）

Effect of Jinzhen oral liquid on the model of human coronavirus pneumonia and fever epidemic immature adult mice

ZHAO Ronghua1, SUN Jing1, BAO Lei1, GENG Zihan1, Li Guiping2,3, WANG Daohan4, LI Shuran1, ZHANG Jingsheng1, PANG Bo1, XU Yingli1, ZHOU Lirun1, BAO Yanyan1, CHEN Mengping1, GUO Shanshan1#, CUI Xiaolan1,*   

  1. 1Biosecurity Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2Jiangsu Kanion Pharmaceutical CO., LTD., Lianyungang Jiangsu 222001, China;
    3State Key Laboratory of New Technology for Pharmaceutical Process of Traditional Chinese Medicine, Lianyungang Jiangsu 222001, China;
    4Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2022-07-01 Online:2023-03-15 Published:2023-03-17

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摘要： 目的 研究金振口服液对人冠状病毒肺炎热毒疫幼龄小鼠模型的干预作用。方法 ICR小鼠,分为空白组,人冠状病毒229E（HCoV-229E）感染组,中医热证组,人冠状病毒肺炎热毒疫小鼠模型组,磷酸氯喹组,连花清瘟组及金振口服液高、中、低剂量组（30、15 和7.5 mL·kg-1·d-1,相当于生药8 、4 和2 g·kg-1·d-1）,每组10只,采用热湿环境复合HCoV-229E感染造模及给药。观察小鼠的肺指数和抑制率,肺组织核酸,ELISA法检测小鼠肺组织因子IL-1β、MDA及下丘脑因子PGE2、CAMP,流式细胞术检测小鼠外周血免疫细胞比例。结果 金振口服液中剂量组可显著降低模型小鼠肺指数（P<0.01）,肺指数抑制率为43.93%;金振口服液高、中剂量组可显著降低模型小鼠肺组织病毒载量、IL-1β含量（P<0.05,P<0.01）、中剂量组可显著降低模型小鼠MDA水平（P<0.01）;金振口服液3个剂量组可显著降低模型小鼠下丘脑中PGE2、CAMP的生成（P<0.05,P<0.01）;对外周血免疫细胞百分比无显著影响。结论 金振口服液可明显改善人冠状病毒肺炎热毒疫幼龄小鼠的免疫功能,表现为通过抑制肺组织中炎性细胞因子表达而缓解肺部炎性损伤,可显著降低模型小鼠肺指数和病毒载量,同时可下调体温调节中枢因子的表达,对模型小鼠的肺部炎症起到干预作用。

关键词: 金振口服液, 人冠状病毒肺炎, 热毒疫, 幼龄小鼠, 炎性因子, 免疫调节, 肺指数, 肺指数抑制率

Abstract: Objective To study the therapeutic effect of Jinzhen oral liquid against a human coronavirus pneumonia and fever epidemic immature adult mouse model. Methods ICR mice were divided into the blank group, HCoV-229E infection group, TCM heat syndrome group, human coronavirus pneumonia and fever epidemic mouse model group, chloroquine phosphate group, Lianhua Qingwen group and Jinzhen oral liquid high-dose, medium-dose and low-dose groups (30 mL·kg-1·d-1, 15 mL·kg-1·d-1, 7.5 mL·kg-1·d-1), with 10 mice in each. HCoV-229E infection was used for modeling and administration. Lung indexes and inhibition rates of mice were observed. The nucleic acid of lungs was detected by ELISA. IL-1β, while MDA and hypothalamus factors PGE2 and CAMP by flow cytometry. Results The lung index of model mice in the Jinzhen oral liquid medium dose group was significantly reduced (P<0.01), and the inhibition rate of the lung index was 43.93%. The viral load and IL-1β in lung tissues of model mice were significantly reduced in the Jinzhen oral liquid high-dose and medium-dose groups (P<0.05, P<0.01). MDA in lung tissues of model mice was significantly reduced in the medium-dose group (P<0.01). The production of PGE2 and CAMP in the hypothalamus of model mice was significantly inhibited in the Jinzhen oral liquid group (P<0.05, P<0.01). The percentage of peripheral blood immune cells was not significantly affected. Conclusion Jinzhen oral liquid can significantly improve the immunity of the coronavirus pneumonia and fever epidemic mouse model by inhibiting the inflammatory cytokine expression in lung tissues, mitigating lung inflammatory injury, reducing significantly the lung index and viral load, and lowering the expression of temperature-regulating central factors.

Key words: Jinzhen oral liquid, human coronavirus pneumonia, fever epidemic, immature adult mouse, inflammatory factors, the immune regulation, the lung index, the inhibition rate of the Lung index

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