中国药物警戒 ›› 2023, Vol. 20 ›› Issue (3): 253-257.
DOI: 10.19803/j.1672-8629.20220367

• 基于感染共性机制的中药防治感染性疾病研究专栏 • 上一篇    下一篇

基于网络药理学及实验验证探究清开灵软胶囊治疗冠状病毒肺炎的作用机制

陈梦苹1,2, 王雅欣2, 姚荣妹2, 包蕾2, 赵荣华2, 孙静2, 耿子涵2, 鲍岩岩2, 戴敏1, 郭姗姗2,*, 崔晓兰2#   

  1. 1安徽中医药大学药学院,安徽 合肥 230012;
    2中国中医科学院中药研究所生物安全实验室,北京 100700
  • 收稿日期:2022-06-30 出版日期:2023-03-15 发布日期:2023-03-17
  • 通讯作者: * 郭姗姗,女,博士,研究员,中药抗病毒及抗感染药理。E-mail: ssguo@icmm.ac.cn;#为共同通信作者。
  • 作者简介:陈梦苹,女,硕士,中药药理。
  • 基金资助:
    国家自然科学基金资助项目（82104500）

Mechanisms of Qingkailing soft capsules against coronavirus pneumonia based on network pharmacology and experimental verification

CHEN Mengping1,2, WANG Yaxin2, YAO Rongmei2, BAO Lei2, ZHAO Ronghua2, SUN Jing2, GENG Zihan2, BAO Yanyan2, DAI Min1, GUO Shanshan2,*, CUI Xiaolan2#   

  1. 1School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei Anhui 230012, China;
    2Biosecurity Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2022-06-30 Online:2023-03-15 Published:2023-03-17

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摘要： 目的 通过网络药理学分析和基于湿热疫毒袭肺病证结合小鼠模型的实验验证,对清开灵软胶囊治疗冠状病毒肺炎的作用机制进行初步探索。方法 从建库开始截至2022年6月28日,通过TCMSP数据库和文献挖掘获得清开灵软胶囊主要活性成分,利用Swiss Target Prediction、GeneCards数据库获得清开灵软胶囊治疗冠状病毒肺炎的潜在靶点,构建PPI互作网络和“药物-成分-靶点”网络,对潜在靶点进行KEGG Pathway富集分析。基于湿热疫毒袭肺小鼠病证结合模型,检测清开灵软胶囊对肺指数的影响,Elisa法检测用药后肺组织中IL-6、SOD和下丘脑中PGE2、CAMP含量的变化。结果 筛选出清开灵软胶囊活性成分78个,潜在靶点112个。“药物-成分-靶点”网络中包含296条边,7个药物节点,槲皮素等33个化合物节点,表皮生长因子受体等112个靶点节点,涉及卡波西肉瘤相关疱疹病毒感染通路、PI3K-AKT等信号通路。与模型组相比,清开灵软胶囊组肺指数明显降低（P<0.01）,肺组织中IL-6、下丘脑中PGE2的含量也显著降低（P<0.01）。结论 清开灵软胶囊可能通过调节炎症介质和发热介质,对冠状病毒肺炎发挥治疗作用。

关键词: 清开灵, 软胶囊, 网络药理学, 人冠状病毒229E, 湿热疫毒袭肺

Abstract: Objective To study the targets and signal pathways of Qingkailing soft capsules in the treatment of coronavirus pneumonia based on network pharmacology. Methods The main chemical ingredients of Qingkailing soft capsules were obtained by searching the TCMSP database for related literature that was published between inception and June 28, 2022. The potential targets of Qingkailing soft capsules in the treatment of coronavirus pneumonia were obtained from Swiss Target Prediction and GeneCards database. A PPI interaction network and “drug-component-target” network were constructed before the potential targets were analyzed via KEGG pathway enrichment. The effect of Qingkailing soft capsules on lung indexes was detected, and the contents of IL-6, SOD in lung tissues and those of PGE2 and CAMP in the hypothalamus were detected by Elisa. Results The “drug-component-target” network consisted of 296 edges, 7 drug nodes, 33 compound nodes and 112 target nodes. Compared with the model group, the lung indexes of the Qingkailing soft capsule group decreased significantly（P＜0.01）, so did the contents of IL-6 in lung tissues and PGE2 in the hypothalamus（P＜0.01）. Conclusion Qingkailing soft capsules may play a role in the treatment of coronavirus pneumonia by regulating inflammatory mediators and febrile mediators.

Key words: Qingkailing, soft capsules, network pharmacology, HCoV-229E, lung-attacking damp-heat epidemic toxin

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