热炎宁合剂对呼吸系统感染性炎症动物抗感染作用研究

doi:10.19803/j.1672-8629.20220290

摘要/Abstract

摘要： 目的观察热炎宁合剂对流感病毒、肺炎链球菌、乙型溶血性链球菌诱导的呼吸系统感染性动物的药效作用。方法分别进行热炎宁合剂（小鼠5、10、20 mL·kg^-1;大鼠2.5、5、10 mL·kg^-1,相当于人用临床剂量0.5倍、等倍及2倍剂量）对流感病毒H1N1/FM1株致小鼠肺炎感染的治疗作用、对流感病毒H1N1/FM1株致小鼠死亡的保护作用、对肺炎链球菌致小鼠肺炎感染的治疗作用、对乙型溶血性链球菌致小鼠死亡的保护作用、对乙型溶血性链球菌致大鼠急性咽炎感染的治疗作用5个试验,测定各组动物的肺指数,记录动物死亡数情况,计算死亡率、死亡保护率、生命延长率以及大鼠的咽部病理情况。结果热炎宁合剂可以明显降低流感病毒H1N1/FM1株致病毒性肺炎小鼠的肺指数,热炎宁合剂10 mL·kg^-1 剂量组肺指数抑制率为61.98%;热炎宁合剂可降低小鼠死亡率,10 、5 mL·kg^-1剂量组可以延长小鼠存活天数。热炎宁合剂20、10 mL·kg^-1剂量组可以明显降低肺炎链球菌致细菌性肺炎小鼠的肺指数,热炎宁合剂大剂量组肺指数抑制率为49.56%。热炎宁合剂3个剂量组均能够显著降低乙型溶血性链球菌感染小鼠所致细菌呼吸性感染的死亡率,10、5 mL·kg^-1剂量组可以延长小鼠存活天数。热炎宁合剂5 mL·kg^-1剂量组能明显改善大鼠咽部病变情况,降低咽部病变评分。结论热炎宁合剂对流感病毒、肺炎链球菌、乙型溶血性链球菌诱导的呼吸系统感染性小鼠感染和乙型溶血性链球菌感染诱导的大鼠呼吸系统感染均有显著的治疗作用,临床使用剂量下效果与磷酸奥司他韦、阿莫西林相当,同时也为新型冠状病毒感染临床治疗提供了新思路。

关键词: 感染性动物模型, 热炎宁合剂, 呼吸系统, 药效, 肺炎, 流感病毒, 肺炎链球菌, 乙型溶血性链球菌

Abstract: Objective To evaluate the pharmacological effects of Reyanning Heji on mouse animal of respiratory infections caused by the influenza virus, Streptococcus pneumoniae and hemolytic streptococcus. Methods Reyanning Heji (5, 10, 20 mL·kg^-1 for mice, 2.5,5,10 mL·kg^-1 for rats, which were half, equivalent to and double the clinical dose for humans) was used to treat the mouse models of pneumonia caused by influenza virus H1N1 / FM1, Streptococcus pneumoniae and hemolytic streptococcus. The lung index, death rate, death protection rate, life extension rate and pharyngeal pathology of the rats in each group were recorded. Results Reyanning Heji could significantly reduce the lung index of mice with viral pneumonia caused by influenza virus H1N1 / FM1. The inhibition rate of the lung index by Reyanning Heji in the medium dose group was 61.98%. Reyanning Heji could reduce the mortality of mice. The high and medium doses of Reyanning Heji could significantly reduce the lung index of mice with bacterial pneumonia caused by Streptococcus pneumoniae. Reyanning Heji could reduce the mortality of mice with bacterial respiratory infections caused by hemolytic streptococcus. The survival of mice in the medium and low dose groups was prolonged. In the low dose group, the pharyngeal lesions of rats were significantly improved while the pharyngeal lesion score was reduced. Conclusion Reyanning Heji has significant therapeutic effects against the mouse models of respiratory infections caused by the influenza virus, streptococcus pneumoniae, and hemolytic streptococcus. The therapeutic effect is equivalent to that of oseltamivir and amoxicillin at the clinical dosage.

Key words: infectious animal model, anti-infection, Reyanning Heji, respiratory system, pharmacological effects, pneumonia, influenza virus, streptococcus pneumonia, hemolytic streptococcus

中图分类号:

R974
R994.11

包蕾, 耿子涵, 郭姗姗, 姚荣妹, 孙静, 崔晓兰. 热炎宁合剂对呼吸系统感染性炎症动物抗感染作用研究[J]. 中国药物警戒, 2023, 20(3): 241-247.

BAO Lei, GENG Zihan, GUO Shanshan, YAO Rongmei, SUN Jing, CUI Xiaolan. Effect of Reyanning Heji on animal models of respiratory infectious inflammation[J]. Chinese Journal of Pharmacovigilance, 2023, 20(3): 241-247.

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