中国药物警戒 ›› 2021, Vol. 18 ›› Issue (12): 1123-1128.
DOI: 10.19803/j.1672-8629.2021.12.05

• 中药治疗病毒性肺炎的药理及机制研究专栏 • 上一篇    下一篇

柴银颗粒对人冠状病毒肺炎湿热疫毒袭肺小鼠病证结合模型治疗及作用机制研究

赵荣华1, 郭姗姗1, 耿子涵1, 孙静1, 时宇静1, 包蕾1, 鲍岩岩1, 高英杰1, 张贵民2,3, 关永霞2,3, 崔晓兰1,*, 王道涵4#   

  1. 1中国中医科学院中药研究所生物安全试验室,北京 100700;
    2鲁南制药集团股份有限公司,山东 临沂 276006;
    3中药制药共性技术国家重点试验室,山东 临沂 276006;
    4北京中医药大学东方医院儿科,北京,100078
  • 收稿日期:2021-05-06 出版日期:2021-12-15 发布日期:2021-12-16
  • 通讯作者: *崔晓兰，女，博士，研究员，中药抗病毒及抗感染药理。E-mail：cuixiaolan2812@126.com. #为共同通信作者。

Effects of Chai Yin Granules on the Mouse Model of the Disease and Syndrome of Human Coronavirus Pneumonia Caused by Lung-attacking Damp-heat Epidemic Virus

ZHAO Ronghua1, GUO Shanshan1, GENG Zihan1, SUN Jing1, SHI Yujing1, BAO Lei1, BAO Yanyan1, GAO Yingjie1, ZHANG Guimin2,3, GUAN Yongxia2,3, CUI Xiaolan1,*, WANG Daohan4#   

  1. 1Biosecurity Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2Lunan Pharmaceutical Group Co. Ltd., Linyi 276006, China;
    3State Key Laboratory of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi Shandong 276006, China;
    4Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2021-05-06 Online:2021-12-15 Published:2021-12-16

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摘要： 目的 观察柴银颗粒对人冠状病毒肺炎湿热疫毒袭肺小鼠病证结合模型的治疗及作用机制研究。方法 通过柴银颗粒（8.8、4.4、2.2 g/kg）对人冠状病毒肺炎湿热疫毒袭肺小鼠病证结合模型的治疗,从证型外观表现、肺部炎症、肺组织中病毒核酸表达量、下丘脑cAMP及PGE-2含量、肺组织炎性因子含量以及外周血中免疫细胞百分比,系统评价柴银颗粒对模型的治疗机制。结果 模型对照组小鼠体质量下降,摄食及饮水量显著减少,嗜卧懒动甚则精神萎靡,毛色潮湿,扎堆少动,反应迟缓,食欲不振,纳呆,大便先期粘腻后期干结;柴银颗粒高、中、低剂量组小鼠上述症状明显改善。与模型对照组比较,柴银颗粒在高、中剂量小鼠肺指数显著降低,但差异无统计学意义（P>0.05）,肺指数抑制率分别为26.49%、21.06%;柴银颗粒高剂量组小鼠肺组织病毒核酸表达量显著降低（P<0.05）;柴银颗粒3个剂量组PGE2及cAMP的含量均显著降低（P<0.05,P<0.01）;柴银颗粒 3个剂量组小鼠肺组织白细胞介素（IL）-1β、白细胞分化抗原（CD14）、氧化应激因子丙二醛（MDA）含量显著降低、小鼠肺组织SOD含量升高（P<0.05,P<0.01）;柴银颗粒低剂量组小鼠外周血CD4+ T/CD8+ T比值显著升高（P<0.05）。结论 柴银颗粒对人冠状病毒肺炎湿热疫毒袭肺小鼠病证结合模型具有显著的治疗作用,通过降低模型小鼠肺指数、降低小鼠肺组织病毒核酸表达量、抑制免疫炎性因子表达,调节下丘脑体温调节中枢因子表达及外周血淋巴细胞免疫功能等方面对模型进行治疗,相关机制研究结果为临床用药提供了客观的试验数据支持。

关键词: 冠状病毒肺炎, 柴银颗粒, 病证结合模型, 免疫功能, 炎性因子

Abstract: Objective To observe the therapeutic effects and mechanism of Chai Yin granules on a mouse model of the disease and syndrome of human coronavirus pneumonia caused by the lung-attacking damp-heat epidemic virus (LDEV).Methods Chai Yin granules (8.8, 4.4, 2.2 g/kg) were used to treat the model of the disease and syndrome of human coronavirus pneumonia caused by LDEV. The mechanism by which Chai Yin granules worked on the model was evaluated in terms of the manifestations of symptoms, lung inflammation, expressions of viral nucleic acid in lung tissue, contents of cAMP and PGE-2 in the hypothalamus, contents of inflammatory factors in lung tissue and the percentage of immune cells in peripheral blood. Results In the model control group, the body mass decreased, the intakes of food and drinking water decreased significantly, lying and inactivity increased until depression occurred in some cases, the hair was wet, the mice huddled in clusters, their reaction was slow, their appetite was poor, the stool was sticky in the first stage and dry in later stages. The above symptoms were significantly improved in high-, medium- and low-dose Chai Yin granule groups. Compared with the model control group, the lung index in high and medium dose groups decreased significantly, but the difference was not statistically significant (P>0.05). The inhibition rates of the lung index were 26.49% and 21.06%, respectively; The expression of viral nucleic acid in lung tissue of mice in the high dose group decreased significantly (P<0.05). The contents of PGE2 and CAMP in the three groups decreased significantly (P<0.05, P<0.01). The contents of IL-1β, CD14 and MDA in lung tissue of mice in the three groups decreased significantly, but the content of SOD increased (P< 0.05, P<0.01). The percentage of CD4 + T / CD8 + T in peripheral blood of mice in the low-dose group increased significantly (P<0.05).Conclusion Chai Yin granules have a significant therapeutic effect against the mouse model of the disease and syndrome of human coronavirus pneumonia caused by the lung-attacking damp-heat epidemic virus by reducing the lung index of model mice, decreasing the expression of virus nucleic acid in lung tissue, inhibiting the expressions of immune inflammatory factors, regulating the expressions of central factors of hypothalamic thermoregulation and the immune function of peripheral blood lymphocytes.

Key words: coronavirus pneumonia, Chai Yin granules, a combined model of the disease and syndrome, immune function, inflammatory factors

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