土茯苓提取物改善抗生素腹泻小鼠肠道屏障的实验研究

doi:10.19803/j.1672-8629.2022.08.10

摘要/Abstract

摘要： 目的探讨土茯苓提取物改善抗生素相关性腹泻小鼠肠道屏障的作用。方法采用盐酸林可霉素灌胃塑造抗生素相关性腹泻小鼠模型（antibiotic-associated diarrhea, AAD）,分为正常组、模型组、枯草杆菌二联活菌颗粒组（3 g·kg^-1）、土茯苓提取物高剂量组（10.88 g·kg^-1）、土茯苓提取物中剂量组（5.44 g·kg^-1）、土茯苓提取物低剂量组（2.72 g·kg^-1）。观察各组小鼠腹泻情况、体质量变化,采集盲肠内容物进行肠道大肠杆菌、肠球菌、双歧杆菌、乳杆菌4种菌的培养和计数,检测血清白蛋白（albumin, ALB）、血清总蛋白（total protein, TP）、肠道分泌型免疫球蛋白（secretory immunoglobulin A, sIgA）水平,HE染色观察肠道病理形态。结果 AAD小鼠一般状态较差,出现腹泻现象。AAD小鼠血清TP、肠道sIgA水平较正常组显著降低,肠道致病菌大肠杆菌和肠球菌数量显著增加,有益菌双歧杆菌和乳杆菌数量显著减少,且肠黏膜萎缩坏死,肠绒毛倒伏、脱落严重。土茯苓提取物可显著增加AAD小鼠体质量,升高血清TP、ALB、肠道sIgA水平。同时可显著抑制大肠杆菌和肠球菌的生长,促进双歧杆菌和乳杆菌的增殖,改善肠绒毛倒伏脱落现象。结论土茯苓提取物可通过保护肠道屏障改善抗生素相关性腹泻。

Abstract: Objective To investigate the protective effects of smilax glabra extract on intestinal barriers in mice with antibiotic-associated diarrhea. Methods Mice were divided into six groups. Amodel of ntibiotic-associated diarrhea mice was induced by ig administration of lincomycin hydrochloride. During the experiment, the incidence of diarrhea and body weight changes of mice in each group were observed, and the colonic contents of mice were collected to culture four floras: Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus. Levels of serum total protein (total protein, TP), albumin and secretory immunoglobulin A (secretory immunoglobulin A, sIgA) were observed. HE staining was used to observe intestinal pathology. Results Levels of STP, albumin, sIgA and numbers of Bifidobacterium and Lactobacillus were decreased significantly compared with the normal group. At the same time, there were some pathological changes in intestinal histology as shown by the increased number of Escherichia coli and Enterococcus. Smilax glabra could raise the levels of STP, albumin and sIgA in antibiotic-associated diarrhea mice, and improve the injured intestinal histology and disordered intestinal flora by decreasing the number of Escherichia coli and Enterococcus faecalis and increasing the number of Bifidobacterium and Lactobacillus. Conclusions milax glabra can improve antibiotic-related diarrhea by protecting the intestinal barriers.

Key words: smilax glabra extract, antibiotic-associated diarrhea, intestinal barrier, intestinal flora, sIgA

中图分类号:

姜卓希, 林志健, 张冰, 王雨, 徐慧哲, 毛秋月. 土茯苓提取物改善抗生素腹泻小鼠肠道屏障的实验研究[J]. 中国药物警戒, 2022, 19(8): 862-867.

JIANG Zhuoxi, LIN Zhijian, ZHANG Bing, WANG Yu, XU Huizhe, MAO Qiuyue. Effect of smilax glabra extract on intestinal barriers in mice with antibiotic-associated diarrhea: an experimental study[J]. Chinese Journal of Pharmacovigilance, 2022, 19(8): 862-867.

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