Study on Antipyretic Effects in Rats and Its Mechanism of Water Extraction Components of Bupleurum

Abstract

Abstract: Objective To study the antipyretic effects of water extraction of Bupleurum components for rat fever model and to investigate the antipyretic mechanism. Methods Rat fever model was established via the back injection of 2, 4-dinitrophenol (DNP). Different doses of water extraction of Bupleurum components were given to rats by gavage. The temperature was detected in anus once every 30 minutes, continuously determined for 4 hours, recorded anal temperature and observed the antipyretic effect. At the same time, the levels of IL-1β, IL-6, TNF-ɑ, PGE₂ in serum were detected. The content of cyclic adenosine phosphate (cAMP) in hypothalamic and plasma as well as the arginine vasopressin (AVP) levels in brain ventral septal area and plasma. Results The modeling was successful because the rectal temperature of rats increased significantly (P<0.001) after injection of 2, 4-dinitrophenol. The high, medium, low dose of water extraction components of Bupleurum all can reduce the temperature and present a dose dependent relationship, and it was also found that they can decrease the levels of IL-1β, IL-6, TNF-ɑ, PGE₂ in serum, the cAMP in hypothalamic and the AVP in brain ventral septal area. However, the content of AVP in plasma was increased, but there was no significant effect on plasma cAMP level. Conclusion Water extraction of Bupleurum components has the antipyretic effect to the rat fever model. The mechanism of antipyretic effect mainly relates to reducing the serum inflammatory mediators of IL-1β, IL-6, TNF-ɑ, PGE₂ in serum and regulating the synthesis and secretion of cAMP and AVP in hypothalamus.

Key words: Bupleurum chinense, antipyretic effect, mechanism

CLC Number:

R965

SHI Liang, ZHANG Zhi-hui, LI Xiao-yu, LV Li-li, SUN Rong. Study on Antipyretic Effects in Rats and Its Mechanism of Water Extraction Components of Bupleurum[J]. Chinese Journal of Pharmacovigilance, 2016, 13(9): 513-516.

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