Research on Determination of Illegal Additive Abietic Acid in Xiaoerhuadu Series Preparations

Abstract

Abstract: Objective To establish a method for detection of illegal additive abietic acid in Xiaoerhuadu series preparations. Methods Illegal additive abietic acid in Xiaoerhuadu series preparations was determined by HPLC-UV method. The HPLC method was carried on C18 column using acetonitrile, tetrahydrofuran and 0.1% formic acid (45:25:30) as the mobile phase to gradient elution, and the flow rate was 1.0 mL·min^-1, the column temperature was 30℃ and the detection wavelength was 241 nm. Then the positive results were confirmed by HPLC-DAD detector and HPLC-MS. Results The linear range of abietic acid was 25.72 ~321.5 ng. The regression equation was Y=0.038 1X+1.698 1 while the correlation coefficient was 0.999 7. The detection limit was 18.4 μg·g^-1. Abietic acid was found in five out of fifty-six batches of samples. Conclusion The method is convenient, sensitive and effective in screening for illegal additive abietic acid in Xiaoerhuadu series preparations.

Key words: Xiaoerhuadu, abietic acid, HPLC

CLC Number:

R917

WU Jia-li, QU Fan-na, ZHANG Qing-bo, BI Xue-yan. Research on Determination of Illegal Additive Abietic Acid in Xiaoerhuadu Series Preparations[J]. Chinese Journal of Pharmacovigilance, 2016, 13(8): 468-471.

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