Qualitative and Quantitative Analysis of Main Characteristic Components of Glycyrrhiza inflate Bat. with Chemometrics

doi:10.19803/j.1672-8629.2021.09.07

Abstract

Abstract: Objective To identify the main characteristic components of Glycyrrhiza inflate Bat. which distinguish this herbal medicine from Glycyrrhiza uralensis Fisch., and to provide reference for quality control of Glycyrrhiza inflate Bat. Methods Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) technology was used to detect the composition of Glycyrrhiza inflate Bat. and Glycyrrhiza uralensis Fisch. Combined with data analysis software, principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA) were conducted to analyze the collected data in order to reveal the difference in compounds between Glycyrrhiza inflate Bat. and Glycyrrhiza uralensis Fisch. A high performance liquid chromatography (HPLC) method was established for the determination of the main characteristic components of Glycyrrhiza inflate Bat. and Glycyrrhiza uralensis Fisch. Results Licochalcone A, licochalcone B, echinatin and glabrol were identified as the main characteristic components of Glycyrrhiza inflate Bat. that were different from those of Glycyrrhiza uralensis Fisch. An HPLC method for simultaneous determination of the above characteristic components was established. Conclusion This method has proved applicable to determination of licochalcone A, licochalcone B, echinatin and glabrol in Glycyrrhiza inflate Bat., thus contributing to quality control of Glycyrrhiza inflate Bat.

Key words: Glycyrrhiza inflate Bat., multi-component quantification, characteristic components, UPLC-Q-TOF-MS/MS, HPLC, chemometrics

CLC Number:

R927.1

CHEN Jia, NIE Lixing, CHENG Xianlong, HU Xiaoru, WEI Feng, MA Shuangcheng. Qualitative and Quantitative Analysis of Main Characteristic Components of Glycyrrhiza inflate Bat. with Chemometrics[J]. Chinese Journal of Pharmacovigilance, 2021, 18(9): 826-830.

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