Abstract: Objective To establish an HPLC method for determining clindamycin in drainage fluid and explore the clinical applicability of clindamycin hemihydrate calcium sulfate bone cement in the local antibacterial drug delivery system. Methods Agilent ZORBAX Extend-C₁₈ (150 mm×4.6 mm, 5 µm) was used as the column. Phosphate buffer (6.8 mg·mL^-1 KH₂PO₄, pH adjusted to 7.5 with 25% KOH) and acetonitrile (55∶45) served as the mobile phase. The detection wavelength was 210 nm and the flow rate was 1.0 mL·min^-1. Results Clindamycin presented a good linear range of 1.48 to 88.90 μg·mL^-1 (r=0.999 7). The average recoveries were 92.90%, 98.03% and 97.13% respectively. The RSD values were less than 2.0% and 3.5%. Conclusion The reversed-phase high performance liquid chromatography method for the determination of concentrations of clindamycin in drainage fluid is simple, sensitive and accurate, which is of referential value for clinical applications of clindamycin semi-water calcium sulfate bone cement.

Key words: HPLC, bone marrow infection, drainage fluid, clindamycin, semi-water calcium sulfate