Differences of chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. by UHPLC-Q-TOF-MS/MS

doi:10.19803/j.1672-8629.2022.09.05

Abstract

Abstract: Objective To identify the differential chemical compounds of Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L., so as to provide reference for quality evaluation of licorice. Methods Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) technology was used to detect the composition of Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L.. Using data analysis software, principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA) were conducted to analyze the collected data in order to reveal the difference in compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L.. Ultra performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-TQ-MS/MS) was conducted to further verify the accuracy of detection of the differential compounds. Results Eleven compounds such as glabridin, neoliquiritin and glabrol were identified as the differential chemical components of Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L.. Conclusion There are obvious differences in some chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L., which can be used for reference in the formulation of quality standards for licorice.

Key words: Glycyrrhiza uralensis Fisch., Glycyrrhiza glabra L., UHPLC-Q-TOF-MS/MS, UHPLC-TQ-MS/MS, PCA, OPLS-DA, differential compounds

CLC Number:

R917

CHEN Jia, NIE Lixing, HU Xiaoru, LIU Wei, WEI Feng, MA Shuangcheng. Differences of chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. by UHPLC-Q-TOF-MS/MS[J]. Chinese Journal of Pharmacovigilance, 2022, 19(9): 954-958.

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