Identification of Chemical Substances in Fuzheng Guben Granules Based on UPLC-ESI-QTOF-MS/MS Technique

doi:10.19803/j.1672-8629.2021.09.08

Abstract

Abstract: Objective To analyze the chemical constituents in Fuzheng Guben granules using ultra performance liquid chromatography electrospray ion source quadrupole time of flight mass spectrometry (UPLC-ESI-QTOF-MS/MS). Methods The chromatographic column of Acquity UPLC HSS T3 C18 (2.1×100 mm, 1.8 μm) was used. The mobile phase was 0.1% formic acid methanol solution (A)-0.1% formic acid aqueous solution (B) in gradient elution, the flow rate 0.2 ml/min, and the column temperature was 40 ℃. The positive and negative ion modes of each component were determined respectively by Xevo G2-XS ESI ion source. According to such parameters as the retention time, molecular accurate mass number and secondary mass spectrum fragments, Unifi software was used to analyze the fragmentation mode of each component, while the reference chemical component database and literature data were used to analyze the detected chemical components. Results Fifty-six compounds were identified, including 29 flavonoids, 9 anthraquinones, 2 stilbene glycosides, 6 terpenes, 3 saponins and 7 other components. Conclusion This method can identify various chemical components of Fuzheng Guben granules quickly and accurately, which provides data for finding effective substances and establishing methods for total quality control.

Key words: Fuzheng Guben granules, UPLC, ESI, QTOF

CLC Number:

R927.2

TONG Liguo, NIU Yanyan, JIA Lili, WANG Ruoyu, SONG Meiqing, XIA Zhaodi, FENG Mali, WANG Xinwen, JI Haijie. Identification of Chemical Substances in Fuzheng Guben Granules Based on UPLC-ESI-QTOF-MS/MS Technique[J]. Chinese Journal of Pharmacovigilance, 2021, 18(9): 831-836.

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