Chinese Journal of Pharmacovigilance ›› 2016, Vol. 13 ›› Issue (6): 338-340.

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Determination of Saikosaponin a, Paeoniflorin and Baicalin in Chaigeqingre Granula by HPLC

ZENG Jun-fen, LU Jian-wu, SONG Jin-chun   

  1. Department of Pharmacy, Renmin Hospital of Wuhan University, Hubei Wuhan 430060, China
  • Received:2016-07-22 Revised:2016-07-22 Online:2016-06-20 Published:2016-07-22

Abstract: Objective To establish a method for the determination of saikosaponin a, paeoniflorin and baicalin in Chaigeqingre granula by HPLC. Methods The chromatographic column was Agilent-C18(150 mm×4.6 mm, 5 μm), using the gradient mobile phase composed of 0.1% H3PO4 (A) -acetonitrile (B), (0~12 min, 15~20% B; 12~30 min, 20~25% B), the flow rate was 1.0 mL·min-1 and the detection wavelength was 210 nm. The temperature of column was 30℃, and the injection volume was 20 μL.Results The linear range of saikosaponin a, paeoniflorin and baicalin was 1.96~31.36 μg·mL-1, 9.4~150.4 μg·mL-1 and 1.52~24.32 μg·mL-1 with average recovery of 97.65% (RSD=1.29%), 103.56% (RSD=1.14%), 102.83% (RSD=1.20%). Conclusion The method is convenient, accurate and reproducible for the determination of saikosaponin a, paeoniflorin and baicalin in Chaigeqingre granula.

Key words: Chaigeqingre granula, saikosaponin a, paeoniflorin, baicalin, HPLC

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