Abstract: ObjectiveTo compare the acute toxicity of the aqueous solution from Psammosilenes radix, Psammosilenes radix with cortex and Psammosilenes cortex, in order to provide a basis for safe and reasonable medication in clinic. MethodsThree samples of Psammosilenes tunicoides were prepared and compared in accordance with classical acute toxicity test. The toxic symptom was observed, and the death and the change of body weight were recorded. The pathological morphology parameters of dead mice with high dose were performed. The experimental data are calculated LD₅₀ with the Bliss method. ResultsThe LD₅₀ (the 95% confidence interval) of Psammosilenes radix, Psammosilenes radix with cortex, Psammosilenes cortex are respectively 4.638 2 (4.004 6~5.439 6) g·kg^-1, 4.847 1 (4.325 1~5.450 8) g·kg^-1, 6.403 2 (5.720 7~7.210 3) g·kg^-1 (crude drug dose). The main toxic target organs are lung, spleen and stomach. ConclusionThe value of LD₅₀ of different fractions in Psammosilenes tunicoides is: Psammosilenes cortex >Psammosilenes radix with cortex> Psammosilenes radix. However, it has slight difference. The process of toxicity in vivo and toxicity mechanism need to be further studied.

Key words: Psammosilenes tunicoides, aqueous solution, acute toxicity