Effects of Kushenin Pellicle on Hypertrophic Scar and TGF-β/Smad Signal Transduction Pathway

Abstract

Abstract: ObjectiveTo investigate the effects of kushenin on hypertrophic scar and its effective mechanism. MethodsHuman hypertrophic scar tissues were subcutaneously transplanted into the back of 30 nude mice to create the animal models of hypertrophic scar. Ten days after the operation, the mice were distributed into the model control, kushenin pellicle and positive medicine group. Drugs were applied 2 times a day for 8 weeks. The growth of the scar was observed and photographed every week, and its size was measured after 10, 35, 49 and 56 d. RT-PCR and Western Blot assessment was used to detect the mRNA and protein expression of TGF-β1 and Smad3. ResultsThe growth of scars in the model control group was more rapid than that of the kushenin pellicle and positive medicine treatment group. The inhibition effect became more obvious with extended medication time. Compared with the model control group, the mRNA and protein expression of TGF-β1 and Smad3 were declined sharply in kushenin pellicle group. ConclusionThe mechanisms of anti-hypertrophic scar effects of kushenin maybe involve inhibiting the TGF-β/Smad signal transduction pathway.

Key words: kushenin, pellicle, hypertrophic scar, TGF-β1, Smad3

CLC Number:

R965
R285.5

WANG Ai-li, GU Yao-hui, HUANG Jing, JIA Qing, CHEN Bo, XU Shun. Effects of Kushenin Pellicle on Hypertrophic Scar and TGF-β/Smad Signal Transduction Pathway[J]. Chinese Journal of Pharmacovigilance, 2016, 13(2): 74-77.

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