Abstract: Objective To establish method for determination of dye auramine O in Gegen Qinlian tablets by LC-MS/MS. Methods Agilent Eclipse plus C18(2.1×100 mm, 1.8μm) was used. A gradient elution program of acetonitrile-0.01 mol·L^-1 ammonium acetate was adopted at the flow rate of 0.3 mL· min^-1. The column temperature was maintained at 35℃ and the sample size was 2 μL. The analyses were detected by an electrospray ionization tandem mass spectrometry with dynamic MRM. Results The results of verification of methodology showed the calibration curve of auramine O was good linear in the range of 0.093 4~1.869 pg (r =0.999 9), and the recovery was 94.38% (RSD=3.60%, n=9). Conclusion The method was convenient, accurate with good precision, reproducibility and stability which has good linear relationship. It can be used for the determination the content of dye auramine O in Gegen Qinlian tablets.

Key words: Gegen Qinlian tablets, LC-MS/MS, dye, auramine O