Risk Assessment and Determination of Hepatotoxic Pyrrolizidine Alkaloids in Senecionis Cannabifolii Herba

doi:10.19803/j.1672-8629.2021.11.09

Abstract

Abstract: Objectiv eTo analyze the contents of hepatotoxic pyrrolizidine alkaloids in Senecionis cannabifolii Herba, and to carry out preliminary risk assessment based on the research results. Methods A UPLC-MS/MS method was established to determine the contents of jacoline, jacoline N-oxide, jacobine, jaconine and jacobine N-oxide. According to the content determination results, the risk was assessed using the margin of exposure (MOE) method. Results The contents of jacoline, jacoline N-oxide, jacobine, jaconine, and jacobine N-oxide in six batches of Senecionis cannabifolii Herba were 0.23-0.85, 0.25-0.98, 0.85-2.65, 0.37-1.85 and 2.32-7.58 μg/g, respectively. The MOE values ranged from 2 131 to 4 845. Conclusion The method established in this study provides data for quality control and safety evaluation of hepatotoxic pyrrolizidine alkaloids in Senecionis cannabifolii Herba.

Key words: Senecionis cannabifolii Herba, hepatotoxic component, pyrrolizidine alkaloids, UPLC-MS/MS, risk assessment

CLC Number:

R927.2

ZAN Ke, ZHOU Ying, PANG Yu, LI Yaolei, WANG Ying, JIN Hongyu, ZUO Tiantian, MA Shuang-cheng. Risk Assessment and Determination of Hepatotoxic Pyrrolizidine Alkaloids in Senecionis Cannabifolii Herba[J]. Chinese Journal of Pharmacovigilance, 2021, 18(11): 1039-1042.

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