Content determination of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Ditong Biyan Shui by UPLC-MS/MS

doi:10.19803/j.1672-8629.20230740

Abstract

Abstract: Objective To determine the contents of aristolochic acid I and aristolochic acid Ⅱ in Ditong Biyan Shui, and to provide reference for quality control. Methods A UPLC-MS/MS method was established to determine the contents of aristolochic acid I and aristolochic acid Ⅱ. An Waters-ACQUI UPLC HSS T3 C₁₈(2.1 mm×100 mm, 1.8 μm) was used as the chromatographic column. The mobile phase was eluted with acetonitrile (A), 0.1% formic acid solution (B) using a gradient elution method. The electrospray spray ion source (ESI) and positive ion multi-reaction monitoring mode were adopted. The contents were calculated using the standard curve method. Results Aristolochic acid I was not detected in 9 samples of 17 batches of Ditong Biyan Shui (detection limit 0.13 pg), but was detected in 8 batches of samples, with the contents ranging from 0.41 to 3.60 ng·mL^-1. Aristolochic acid Ⅱ was not detected in any of the samples (detection limit 0.41 pg). Conclusion A UPLC-MS/MS method has been established to determine the contents of aristolochic acid I and aristolochic acid Ⅱ in Ditong Biyan Shui, with high specificity, high sensitivity, good repeatability, which can provide reference for quality control of aristolochic acid I and aristolochic acid Ⅱ in Ditong Biyan Shui.

Key words: Ditong Biyan Shui, aristolochic acid I, aristolochic acid Ⅱ, UPLC-MS/MS, content determination

CLC Number:

R927.1

GUO Rixin, XIAO Meng, LIU Jing, DAI Zhong, MA Shuangcheng. Content determination of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Ditong Biyan Shui by UPLC-MS/MS[J]. Chinese Journal of Pharmacovigilance, 2024, 21(4): 386-390.

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