Abstract: Objective To investigate the mechanism of Qingkailing Injection in the treatment of coronavirus pneumonia based on mouse model combining disease and syndrome of human coronavirus pneumonia with heat-dampness pestilence attacking the lung.Methods BALB/c mice were randomly divided into eight groups, including normal group, HCoV-229E infection group, Heat-dampness group, model group, positive drug group（0.09g/(kg·d)）, Qingkailing Injection high-dose group（1.47mL/(kg·d)）, middle-dose group（0.73mL/(kg·d)）and low-dose group（0.37mL/(kg·d)）. The viral load in lung tissue was detected by RT-PCR, the febrile mediators, inflammatory factors and oxidative stress factors in hypothalamus and lung tissue were detected by ELISA, the percentage of immune cells in peripheral blood was detected by flow cytometry. Results Compared with the model, middle-dose and low-dose groups of Qingkailing Injection can reduce the lung index (P<0.05, P<0.01) and the viral load in lung tissue (P<0.01, P<0.05). Three dose groups of Qingkailing Injection reduce the contents of CD14 and IL-1β in lung tissue (P<0.01), the middle- and low-dose groups reduce the content of MDA (P<0.01, P<0.05), the high-dose and middle-dose groups increase the content of SOD (P<0.01), and the three dose groups of Qingkailing Injection could reduce the proportion of CD4⁺ T cells, CD8⁺ T cells and B cells in peripheral blood (P<0.01, P<0.05).Conclusion Qingkailing Injection can play a therapeutic role in this model by regulating immune function, inhibiting cytokine storm and reducing the accumulation of free radicals.

Key words: Qingkailing injection, lung-attacking damp-heat pestilence, HCoV-229E, cytokine, immune regulation