Effects of Emodin-8-O-β-D-glucopyranoside on Tumor Cell Migration and Metastasis in Vitro and in Vivo

doi:10.19803/j.1672-8629.2019.12.01

Abstract

Abstract: Objective To investigate the effects of emodin-8-O-β-D-glucopyranoside (EG) on tumor cell migration in vitro and tumor metastasis of tumor-bearing mice in vivo. Methods Different kinds of cells (mouse breast cancer cell 4T1-Luc, human colorectal cancer cell HCT116 and human neuroblastoma cell SH-SY5Y) were treated with 0, 100, 200, 400 mg·mL^-1 EG while cell proliferation, cell migration and metastasis ability were detected by MTT assay, wound healing and Transwell assay respectively. Then, orthotopic 4T1-Luc bearing mice were treated with phosphate-buffer saline (PBS) and EG at low (2 mg·kg^-1) and high (4 mg·kg^-1) doses for 13 days respectively. Tumor metastasis was observed by the animal imaging system every two days during the period. Results EG could inhibit tumor cell migration in a concentration- and time-dependent manner in these three kinds of cells. It also inhibited tumor cell metastasis in a concentration-dependent manner. In the animal study, EG could also inhibit tumor metastasis in 4T1-Luc bearing mice. Conclusion EG has the ability to inhibit tumor cell migration and metastasis both in vitro and in vivo.

Key words: emodin-8-O-β-D-glucopyranoside, tumor, migration, metastasis

CLC Number:

R965

LI Yiqun, LIANG Huanxi, LIU Changzhen, SUN Zhenxiao. Effects of Emodin-8-O-β-D-glucopyranoside on Tumor Cell Migration and Metastasis in Vitro and in Vivo[J]. Chinese Journal of Pharmacovigilance, 2019, 16(12): 705-710.

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