石斛碱抗脑缺血再灌注损伤的网络药理学研究

doi:10.19803/j.1672-8629.2022.03.05

中国药物警戒 ›› 2022, Vol. 19 ›› Issue (3): 252-258.
DOI: 10.19803/j.1672-8629.2022.03.05

石斛碱抗脑缺血再灌注损伤的网络药理学研究

刘晓莉¹, 刘胜伟², 廖原³, 曾凡平², 刘洁^4,*

¹重庆市永川区中医院内科,重庆 402160;
²重庆医科大学附属永川医院,重庆 402160;
³杭州师范大学生命与环境科学学院,浙江杭州310036;
⁴重庆市江津区中心医院,重庆 402260

收稿日期:2021-12-09 出版日期:2022-03-15 发布日期:2022-03-16
通讯作者: ^*刘洁,女,硕士研究生,主管药师,中药药理与神经药理学。E-mail：453039683@qq.com
作者简介:刘晓莉,女,本科,主治医师,内科学与生物信息学。
基金资助:
国家自然科学基金青年科学基金项目（31701189）; 重庆市科卫联合中医药技术创新与应用发展项目（2021ZY3591）; 重庆医科大学附属永川医院资助项目（YJJC202009）

Network pharmacology of dendrobium alkaloids against cerebral ischemia-reperfusion injury

LIU Xiaoli¹, LIU Shengwei², LIAO Yuan³, ZENG Fanping², LIU Jie^4,*

¹Department of internal medicine, Chongqing Yongchuan District Hospital of Traditional Chinese Medicine, Chongqing 402160, China;
²Yongchuan Hospital of Chongqing Medical University, Chongqing 402160, China;
³College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou Zhejiang, 310036, China;
⁴Jiangjin Central Hospital of Chongqing, Chongqing 402260, China

Received:2021-12-09 Online:2022-03-15 Published:2022-03-16

摘要/Abstract

摘要： 目的基于网络药理学研究石斛碱抗脑缺血再灌注损伤（CIRI）的作用机制。方法通过数据库Pharmmapper、BATMAN-TCM和GeneCards分别对石斛碱及疾病靶点进行预测,利用VENNY2.1.0进行映射得到石斛碱作用于CIRI的潜在靶点,再导入Cytoscape3.8.2软件构建蛋白-蛋白交联互作网络（PPI）。利用R语言的clusterProfiler软件包对石斛碱抗脑缺血再灌注损伤的潜在靶点进行基因本体(Gene Ontology, GO)富集分析和京都基因及基因组百科全书（KEGG）富集通路分析。结果找到石斛碱相关靶点214个,脑缺血再灌注损伤相关靶点513个,交集靶点58个,PPI分析度值排名前10的核心靶基因有ALB、MAPK1、MAPK8、CASP3、IGF1、SRC、NOS3、MMP2、HSP90AA1和ESR1。通过KEGG富集通路分析得到14条显著通路,与相关文献检索报道有关的为Rap1信号通路、MAPK信号通路、趋化因子（chemokine）信号通路等。结论石斛碱通过多靶点、多途径治疗CIRI,其可能主要通过炎症、凋亡等途径起到抗CIRI的作用。

关键词: 石斛碱, 脑缺血再灌注, 网络药理学

Abstract: Objective To study the mechanism by which dendrobium alkaloids combats cerebral ischemia-reperfusion injury based on network pharmacology. Methods The databases Pharmamapper and BATMAN-TCM were used to predict dendrobium alkaloids targets, and disease targets associated with cerebral ischemia-reperfusion injury (CIRI) were acquired via the GeneCards database. VENNY2.1.0 was used to map the potential targets of dendrobium alkaloids acting on CIRI before the potential targets were imported to Cytoscape3.8.2 software so as to construct a PPI network. Finally, the R package clusterProfiler was used for GO enrichment analysis and KEGG pathway analysis. Results A total of 214 dendrobine-related targets and 513 cerebral ischemia-reperfusion injury-related targets were found, 58 of which were intersection targets. After PPI analysis, the top ten core target genes were ALB, MAPK1, MAPK8, CASP3, IGF1, SRC, NOS3, MMP2, HSP90AA1 and ESR1. Based on the analysis of KEGG enrichment pathways, 14 significant pathways were obtained, including the Rap1 signaling pathway, MAPK signaling pathway and chemokine signaling pathway. Conclusion Dendrobium alkaloids can treat CIRI through multiple targets and multiple pathways, and may play a role in resisting cerebral ischemia-reperfusion injury mainly by means of inflammation and apoptosis.

Key words: dendrobium alkaloids, cerebral ischemia-reperfusion injury, network pharmacology

中图分类号:

R966R285

刘晓莉, 刘胜伟, 廖原, 曾凡平, 刘洁. 石斛碱抗脑缺血再灌注损伤的网络药理学研究[J]. 中国药物警戒, 2022, 19(3): 252-258.

LIU Xiaoli, LIU Shengwei, LIAO Yuan, ZENG Fanping, LIU Jie. Network pharmacology of dendrobium alkaloids against cerebral ischemia-reperfusion injury[J]. Chinese Journal of Pharmacovigilance, 2022, 19(3): 252-258.

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石斛碱抗脑缺血再灌注损伤的网络药理学研究

Network pharmacology of dendrobium alkaloids against cerebral ischemia-reperfusion injury

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