Study on Correlationship between Activity of T Lymphocyte Subsets and NK Cells and Activity of Probiotics in Cancer Patients

Abstract

Abstract: Objective To study the correlationship between the activity of T lymphocyte subsets and NK cells and the activity of probiotics in cancer patients. Methods The activity of T lymphocyte subsets and NK cells in the peripheral blood of cancer patients and the healthy controls were detected by using flow cytometry. The total number of bacteria and the number of lactobacillus in the feces of cancer patients and the healthy controls were detected by using plate count method. The correlationship between the activity of probiotics, T lymphocyte subsets, and NK cells and antibiotics use in cancer patients was statistically analyzed by t-test of two sample mean,one-way analysis of variance(F-test), q-test and correlation analysis, and the correlation coefficients were also examined. Results In overall comparison of cell detecting, the percentage of CD3+, CD4+, NK cells, and CD4+/CD8+ ratio in peripheral blood of cancer patients decreased significantly, while the percentage of CD8+ significantly increased, compared with the normal control group, the difference was statistically significant(each P< 0.05). In stratified comparison of cell detecting, there was no statistical difference in the percentage of CD3+, CD4+, NK cells, and CD4+/CD8+ ratio in peripheral blood between the fewer days group and the control group(each P> 0.05). Compared with the normal control group, both the moderate days group and the more days group had statistical difference in the percentage of CD3+, CD4+, CD8+, NK cells , and CD4+/CD8+ ratio in peripheral blood(each P< 0.05; 0.01). In overall comparison of bacteria detecting, The total number of bacteria of the case group increased significantly,while the number of lactobacillus significantly decreased, compared with the normal control group, the difference was statistically significant(each P< 0.050). In stratified comparison of bacteria detecting, there was no statistical difference in the total number of bacteria and the number of lactobacillus between the fewer days group and the normal control group(each P> 0.05). Compared with the normal control group,both the more days group and the moderate days group had statistical difference in the total number of bacteria and lactobacillus(P< 0.05, P<0.01). with the correlation analysis, there was a weakly positive correlation between the activity of CD3+, CD4+, CD8+, NK cells, and CD4+/CD8+ ratio in peripheral blood and the activity of lactobacillus of the cancer patients(r=0.385, 0.406, 0.371, 0.383, 0.401, each P< 0.05). Conclusion There is a weakly positive correlation between the activity of T lymphocyte subsets, NK cells and the activity of probiotics in cancer patients.

Key words: cancer, probiotics, lactobacillus, T lymphocyte subsets, NK cells, activity, antibiotics, correlationship

CLC Number:

R969.3

ZHANG Hong-xu, LIU Guang-feng, HAN Feng, HUANG Jin-yue, LING Xin, WANG Yan. Study on Correlationship between Activity of T Lymphocyte Subsets and NK Cells and Activity of Probiotics in Cancer Patients[J]. Chinese Journal of Pharmacovigilance, 2016, 13(4): 206-211.

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