HT22 Cell and Caenorhabditis elegans models of the anti-aging effect of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair

doi:10.19803/j.1672-8629.20230111

Abstract

Abstract: Objective To preliminarily discuss the anti-aging effect of Ginseng Radix et Rhizoma Rubra(GR) and Polygoni Multiflori Radix Preparata (PMRP) on nerve cells under different extraction processes, and provide data support for the application of GR and PMRP in anti-aging and anti-Alzheimer's disease (AD). Methods GR and PMRP medicinal pair extracts of 30% ethanol reflux, 30% ethanol percolation, 70% ethanol reflux, 70% ethanol percolation and water extract were prepared. The effects of different extracts on β-Galactosidase content in HT22 cells were detected by flow cytometry, and the extracts with the best anti-aging effect on nerve cells were initially screened. The anti-aging effect of the extracts on nerve cells was directly analyzed by β-galactosidase staining, and the anti-aging and anti-AD effects were verified by nematode life test and paralysis experiment. Results The different extraction processes of GR and PMRP showed significant anti-aging effects on HT22 cells, and 70% ethanol percolation extract had the best effect. Multi-concentration validation showed that it had a dose-dependent protective effect. The 70% ethanol percolation extract at 0.2 mg•mL^-1 could significantly prolong the life of Caenorhabditis elegans, and the 70% ethanol percolate extract at 0.008 mg•mL^-1 showed a tendency to relieve paralysis. Conclusion The 70% ethanol percolation extract of GR and PMRP has obvious anti-aging effect on nerve cells, has the potential of anti-aging and prolonging life and treating AD, which should be further studied.

Key words: Ginseng Radix et Rhizoma Rubra and Polygoni Multiflori Radix Preparata medicinal pair, anti-aging, HT22 cells, Caenorhabditis elegans, Alzheimer's disease, safety, life experiment, beta-galactosidase

CLC Number:

R917

LIU Jingjing, XU Beilei, YANG Jianbo, WANG Ying, LIU Yue, WANG Qi, MA Shuangcheng. HT22 Cell and Caenorhabditis elegans models of the anti-aging effect of Ginseng Radix et Rhizoma Rubra and Polygonum Multiflorum Radix preparata medicinal pair[J]. Chinese Journal of Pharmacovigilance, 2023, 20(6): 629-633.

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