Study on the components of Polygonum multiflorum aqueous extract in rats after long-term administration

doi:10.19803/j.1672-8629.2022.06.08

Abstract

Abstract: Objective To investigate the accumulation characteristics of the components in the liver, kidney, plasma and bile of rats after gavage of the aqueous extract of Polygonum multiflorum for 42 consecutive days, so as to provide data support for the study on the hepatotoxicity of Polygonum multiflorum. Methods UPLC-LTQ-Orbitrap MS technology was applied to qualitatively identify and speculate the prototype components and metabolites of Polygonum multiflorum in different tissues of rats by comparing with the reference substance and analyzing using the scitific library module. Results After a long-term induction with the aqueous extract of Polygonum multiflorum by oral gavage in the rat, tweenty-four related compounds of Polygonum multiflorum could be identified and detected in vivo, of which 7 were prototype components, 5 were phase Ⅰ metabolites, and 12 were phase Ⅱ metabolites. Among which, the main components included stilbene glycosides and anthraquinone prototypes and their metabolites. Various components had a selective accumulation pattern, and the most of them accumulated in the kidney, the contents in the liver and bile were equivalent, and the least was found in the plasma. Stilbene glycosides were mainly subjected to sulfation and glucuronidation reactions, while emodin mostly undergoes oxidation reactions and glucuronidation reactions. Conclusion The prototype components emodin, aloe-emodin, emodin-8-O-glucoside, stilbene glycosides, rhein, and gallic acid are likely to be highly related to the adverse reactions caused by Polygonum multiflorum, which should attract attention. Acid and stilbene glycoside-glucuronide-sulfate are only detected in bile, gallic acid and protocatechuic acid and a large number of emodin phase II metabolites are only detected in kidney, while other potential toxicity risks should be further investigated.

Key words: Polygonum multiflorum, aqueous extract of polygonum multiflorum, rats, gavage, tissue distribution, metabolites, safety evaluation

CLC Number:

R961
R962

WANG Qi, WEN Hairuo, YANG Jianbo, WANG Ying, LI Yanyi, MA Shuangcheng, ZHANG Yujie. Study on the components of Polygonum multiflorum aqueous extract in rats after long-term administration[J]. Chinese Journal of Pharmacovigilance, 2022, 19(6): 620-625.

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