Inhibitory effect of total saponins from Kangke capsules on apoptosis induced by Coxsackie virus B

doi:10.19803/j.1672-8629.2022.04.09

Abstract

Abstract: Objective To observe the inhibitory effects of saponins from Kangke capsules on apoptosis caused by Coxsackievirus B (CVB), and to explore the mechanism. Methods CVB was inoculated into cardiomyocytes to establish an infection model in vitro. The cytopathic effect method (CPE) was adopted to determine the 50% inhibitory concentration (IC₅₀) and therapeutic index (TI) of saponins from Kangke capsules on CVB₃, CVB₄ and CVB₅. CCK-8 kit was used to detect cell viability. TdT-mediated dUTP notch end labeling technique (TUNEL) was used to determine the apoptosis rate of cardiomyocytes. Western-blot was used to study the expressions of Bcl-2 and Bax protein in primary rat cardiomyocytes. Results Saponins from Kangke capsules could significantly alleviate cytopathy induced by CVB. The IC₅₀ for CVB₃, CVB₄, and CVB₅ was 223.34, 315.48 and 306.53 μg·mL^-1 respectively, while the therapeutic index was 4.68, 3.24 and 3.24 respectively. The doses of 390.63, 195.32, and 97.66 μg·mL^-1 could increase the survival rate of cardiomyocytes after viral infection. Saponins from Kangke capsules could significantly inhibit viral replication and cell apoptosis after infection while increasing the expression of Bcl-2, decreasing the expression of Bax, and elevating the Bcl-2/Bax ratio. Conclusion Saponins from Kangke capsules can improve cytopathy caused by CVB infection, improve cell survival rate, and inhibit apoptosis. The mechanism is related to the regulation of apoptosis-related protein expressions in cardiomyocytes.

Key words: saponins from Kangke capsules, Coxsackievirus B, cardiomyocytes, apoptosis

CLC Number:

R978.7

ZHANG Zhibin, YAO Rongmei, MA Huixia, BAO Jutai, GUO Shanshan, CUI Xiaolan. Inhibitory effect of total saponins from Kangke capsules on apoptosis induced by Coxsackie virus B[J]. Chinese Journal of Pharmacovigilance, 2022, 19(4): 384-389.

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