Variations of chemical substances and toxic substances of Evodia rutaecarpa using different extraction methods

doi:10.19803/j.1672-8629.20210518

Abstract

Abstract: Objective To study the chemical substances of Evodia rutaecarpa extracted using different methods and the possible main material basis of hepatotoxicity. Methods The water extract, 30% ethanol extract, 50% ethanol extract and 80% ethanol extract of Evodia rutaecarpa were prepared respectively. The differential chemical substances were identified using the method of phytomics, and the toxicity of the main differential chemical substances was screened via software prediction. Results The results showed that there were 50 types of different chemical substances in water extraction and ethanol extraction of Evodia rutaecarpa, among which the most important one was Evodia alkaloids. The results of toxicity endpoint screening showed that there were safety risks in Evodia rutaecarpa alkaloids. The toxicity of Evodia rutaecarpa alcohol extract was higher than that of water extract. Conclusion Different extraction methods can make a difference to chemical substances of Evodia rutaecarpa, and Evodia alkaloids may be the basis of its toxic substances .

Key words: Evodia rutaecarpa, phytomics, toxic substance basis, software prediction

CLC Number:

R927

JI Hainan, TONG Na, LIU Chang, ZHAO Chan, ZHOU Xiaojuan, SUI Feng, SHEN Guolin. Variations of chemical substances and toxic substances of Evodia rutaecarpa using different extraction methods[J]. Chinese Journal of Pharmacovigilance, 2022, 19(12): 1309-1314.

References

[1] Pharmacopoeia of the People’s Republic of China (Volume I)(中华人民共和国药典一部,2020年版)[M]2020年版)[M]. Beijing: China Medical Science Press, 2020: 178.
[2] JIA S, HU C.Pharmacological effects of rutaecarpine as acardiovascular protective agent[J]. Molecules, 2010, 15(3): 1873-1881.
[3] ZHANG B.Characteristics and safety of chinese functional food materials[J]. Food Science(食品科学), 2011, 32(21): 298-300.
[4] LI YJ, ZHANG F, GONG QH, et al.Rutaecarpine inhibits angiotensin II-induced proliferation in rat vascular smooth muscle cells[J]. Chin J Integr Med, 2014, 20(9): 682-687.
[5] WEN LM, MA CY, YU DL.Research progress on chemical constituents and pharmacological effects of Evodia rutaecarpa[J]. Chinese Journal of Traditional Chinese Medicine(中华中医药学刊), 2012, 30(9): 1976-1977.
[6] CAI XY, MENG N, YANG B.Analysis of a case of poisoning caused by taking Evodia rutaecarpa[J]. Beijing Traditional Chinese Medicine(北京中医药), 2006, 25(3): 171-172.
[7] CHEN YY.Talking about the poisoning and treatment of Evodia rutaecarpa[J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine(浙江中西医结合杂志), 1999, 9(4): 281.
[8] LI L, ZHAO JN, YI JH.Study on toxic effects of Evodia rutaecarpa from multiple sources and multiple habitats[J]. Chinese Journal of Traditional Chinese Medicine(中国中药杂志), 2012, 37(15): 2220-2221.
[9] ZHOU Q, ZHANG X, JIN RM.Study on the time-effect and dose-effect relationship of mouse hepatotoxicity induced by Evodia rutaecarpa[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2011, 17(9): 232-235.
[10] LI L, ZHAO JN, YAN LC.Long-term toxicity test of Evodia rutaecarpa water extract in rats[J]. Pharmacology and Clinical of Traditional Chinese Medicine(中药药理与临床), 2013, 29(2): 93-96.
[11] ZHU LL, YANG DX, LIU X.Study on hepatotoxicity of Evodia rutaecarpa fruit and preliminary exploration of toxic parts[J]. Shizhen Chinese Medicine(时珍国医国药), 2013, 24(8): 1810-1811.
[12] HUANG W, ZHAO Y, SUN R.Comparative study on acute toxicity test of different components of Evodia rutaecarpa in mice[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2010, 7(3): 129-134.
[13] YUAN XL, ZHANG P, LIU XM, et al.Cytological assessments and transcriptome profiling demonstrate that evodiamine inhibits growth and induces apoptosis in a renal carcinoma cell line[J]. Scientific Reports, 2017, 7(1): e12572.
[14] LIN L, REN L, WEN L, et al.Effect of evodiamine on the proliferation and apoptosis of A549 human lung cancer cells[J]. Molecular Medicine Reports, 2016, 14(3): 2832-2838.
[15] HAN MY, HU L, CHEN Y.Rutaecarpine may improve neuronal injury, inhibits apoptosis, inflammation and oxidative stress by regulating the expression of ERK1/2 and Nrf2/HO-1 pathway in rats with cerebral ischemia-reperfusion injury[J]. Drug Design, Development and Therapy, 2019, 13: 2923-2931.
[16] WANG CH, HAO ZY, ZHOU J.et al.Rutaecarpine alleviates renal ischemia reperfusion injury in rats by suppressing the JNK/p38 MAPK signaling pathway and interfering with the oxidative stress response[J]. Molecular Medicine Reports, 2017, 16: 922-928.
[17] ZENG SY, YANG L, LU HQ, et al.Rutaecarpine prevents hypertensive cardiac hypertrophy involving the inhibition of Nox4-ROS-ADAM17 pathway[J]. Journal of Cellular and Molecular Medicine, 2019, 23: 4196-4207.
[18] ZHOU Q, JIN RM, YAO GT.Preliminary study on renal cytotoxicity caused by four monomer components in Evodia rutaecarpa[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2013, 10(1): 1-5.
[19] YANG XW.Acute toxicity and genotoxicity test of water and 70% ethanol extract of Evodia rutaecarpa[J]. Chinese Journal of Traditional Chinese Medicine(中国中药杂志), 2008, 33(11): 1317-1321.
[20] ZHANG X, ZHOU Q, JIN RM.Preliminary study on hepatotoxicity of rutaecarpine[J]. Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志), 2011, 17(8): 221-225.