中国药物警戒 ›› 2023, Vol. 20 ›› Issue (7): 769-774.
DOI: 10.19803/j.1672-8629.20220715

• 基础与临床研究 • 上一篇    下一篇

UPLC-PDA切换波长法测定柴胡-白芍药对水煎液7种主要成分的含量

吴玲芳1,2,3, 田伟1,2,3, 马永犇1,2,3, 高乐1,2,3, 孙铮1, 王鑫国1,2,3, 牛丽颖1,2,3*   

  1. 1河北中医学院,河北 石家庄 050091；
    2河北省中药配方颗粒技术创新中心,河北 石家庄 050091；
    3中药材品质评价与标准化河北省工程研究中心,河北 石家庄 050091
  • 收稿日期:2022-12-12 出版日期:2023-07-15 发布日期:2023-07-14
  • 通讯作者: *牛丽颖,女,硕士,教授,中药分析与药效物质基础研究。E-mail: niuliyingyy@163.com
  • 作者简介:吴玲芳,女,博士,副教授,中药药效物质研究。
  • 基金资助:
    河北省自然科学基金重点项目(H2022423335); 河北省重点研发计划项目（20372502D）; 河北省高等学校科学技术研究资助项目(QN2019119); 河北省中医药管理局科研计划项目(2020130); 河北中医学院博士基金资助项目(BZS2019001)

Determination of seven main components in aqueous extract of Bupleuri Radix-Paeoniae Alba Radix by UPLC-PDA

WU Lingfang1,2,3, TIAN Wei1,2,3, MA Yongben1,2,3, GAO Le1,2,3, SUN Zheng1, WANG Xinguo1,2,3, NIU Liying1,2,3,*   

  1. 1Hebei University of Traditional Chinese Medicine, Shijiazhuang Hebei 050091, China;
    2Hebei Traditional Chinese Medicine Formula Granule Engineering & Technology Innovation Center, Shijiazhuang Hebei 050091, China;
    3Hebei Engineering Research Center for Quality Evaluation & Standardization of Traditional Chinese Medicine, Shijiazhuang Hebei 050091, China
  • Received:2022-12-12 Online:2023-07-15 Published:2023-07-14

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摘要： 目的 建立超高效液相色谱-二极管阵列检测器（UPLC-PDA）切换波长法同时测定柴胡-白芍药对水煎液中7种主要成分没食子酸、芍药内酯苷、芍药苷、苯甲酰芍药苷、柴胡皂苷A、柴胡皂苷C和柴胡皂苷D含量的方法。方法 采用 Waters ACQUITY UPLC BEH C18（2.1 mm×100 mm, 1.7 μm）色谱柱,流动相：乙腈-0.1%乙酸水溶液,梯度洗脱;流速为 0.2 mL·min-1;检测波长分别为210（柴胡皂苷A、C、D）、237（芍药内酯苷、芍药苷、苯甲酰芍药苷）、278 nm（没食子酸）,柱温30℃;进样量8 μL。结果 没食子酸、芍药内酯苷、芍药苷、苯甲酰芍药苷、柴胡皂苷A、柴胡皂苷C和柴胡皂苷D的线性范围分别为0.018~0.18 μg（r=0.999 9）、0.047~0.47 μg（r=1）、0.52~5.18 μg（r=0.999 9）、0.021~0.21 μg（r=0.999 9）、0.043~0.43 μg（r=0.999 9）、0.073~0.73 μg（r=0.999 9）、0.042~0.42 μg（r=0.999 6）,平均加样回收率（n=9）均在99.97%~101.47%,相对标准偏差（RSD）均小于2%。结论 所建立的多成分含量测定方法快捷、准确、重复性好,可用于柴胡-白芍药对水煎液的质量控制。

关键词: 柴胡-白芍, 芍药苷, 柴胡皂苷A, 药对, 超高效液相色谱-二极管阵列检测器, 含量测定

Abstract: Objective To establish a method for simultaneous determination of gallic acid, paeoniflorin, albiflorin, benzoyl paeoniflorin, saikosaponin A, saikosaponin C and saikosaponin D in the aqueous extract of Bupleurum and Paeoniae Alba by UPLC-PDA. Methods The Waters ACQUITY UPLC BEH C18 (2.1 mm×100 mm, 1.7 μm) column was used with the mobile phase composed of acetonitrile - 0.1% acetic acid aqueous solution under gradient elution. The flow rate was 0.2 mL·min-1 at detection wavelengths of 210 nm for saikosaponin A, saikosaponin C and saikosaponin D, 237 nm for paeoniflorin, albiflorin and benzoyl paeoniflorin, and 278 nm for gallic acid. The column temperature was 30℃ while the injection volume was 8 μL. Results The linear ranges of gallic acid, paeoniflorin, albiflorin, benzoyl paeoniflorin, saikosaponin A, saikosaponin C and saikosaponin D were 0.018~0.18 μg (r=0.999 9), 0.047~0.47 μg (r=1), 0.52~5.18 μg (r=0.999 9), 0.021~0.21 μg (r=0.999 9), 0.043~0.43 μg (r=0.999 9), 0.073~0.73 μg (r=0.999 9), and 0.042~0.42 μg (r=0.999 6) respectively. The average recovery (n=9) ranged from 99.94 to 101.47%, and the RSD was less than 2%. Conclusion This method is rapid, accurate and reproducible. It can be used for quality control of decoction of Radix Bupleuri-Paeoniae Alba.

Key words: bupleuri radix-paeoniae alba radix, paeoniflorin, saikosaponin A, herb pair, UPLC-PDA, determination

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