中国药物警戒 ›› 2022, Vol. 19 ›› Issue (10): 1060-1066.
DOI: 10.19803/j.1672-8629.20210403

• 基础与临床研究 • 上一篇    下一篇

基于RGB多通道图像数据的牛胆与羊胆薄层色谱特征分析

石岩, 魏锋*, 马双成#   

  1. 中国食品药品检定研究院,北京102629
  • 收稿日期:2021-04-26 出版日期:2022-10-15 发布日期:2022-10-17
  • 通讯作者: *魏锋,男,博士,研究员,中药质量评价与控制。E-mail: weifeng@nifdc.org.cn #为共同通信作者。
  • 作者简介:石岩,男,博士,研究员,中药质量评价与控制。
  • 基金资助:
    国家重点研发计划( 2019YFC1711500); 重大新药创制国家科技重大专项2018年度(2018ZX09735-006)

The TLC characteristic analysis of cattle bile and sheep bile based on RGB multichannel data of images

SHI Yan, WEI Feng*, MA Shuangcheng#   

  1. National Institutes for Food and Drug Control, Beijing 102629, China
  • Received:2021-04-26 Online:2022-10-15 Published:2022-10-17

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摘要： 目的 旨在对牛胆和羊胆药材进行薄层色谱（thin-layer chromatography, TLC）特征性鉴别研究。方法 使用异辛烷-乙醚-冰醋酸-正丁醇-水（6:5:5:3:1）为展开剂,10%硫酸乙醇溶液为显色剂,105 ℃加热至斑点清晰,366 nm下检识并拍照储存。对样品的TLC彩色图像RGB多通道数据进行提取,并应用层次聚类分析（hierarchical cluster analysis, HCA）、主成分分析（principal component analysis, PCA）以及正交偏最小二乘判别分析（orthogonal projections to latent structures - discrimination analysis, OPLS-DA）对数据进行分析。结果 各通道数据以B通道数据最具有差异特征性,而甘氨胆酸、甘氨去氧胆酸以及第4个斑点为最具特征性的色谱斑点。结论 相较于传统TLC鉴别方法,所建立的分析方法简便、灵敏准确,可以为其他TLC鉴别研究提供参考。

关键词: 牛胆, 羊胆, 薄层色谱, 鉴别, 化学计量学

Abstract: Objective To investigate the Thin-layer Chromatography (TLC) characteristic of cattle bile and sheep bile. Methods TLC method was developed with isooctane-ether-glacial acetic acid-n-butanol-water (6:5:5:3:1) as developer, 10% sulfuric acid ethyl alcohol solution as chromogenic reagent. After heating at 105 ℃ until spots marked, TLC was checked and stored as a image. Then RGB multichannel data of TLC image were extracted out and analyzed by Hierarchical Cluster Analysis (HCA), Principal Components Analysis (PCA) and Orthogonal Projections to Latent Structures - Discrimination Analysis (OPLS-DA). Results The data of channel of B had the most characteristic for the difference of cattle bile and sheep bile. In TLC, spots of glycocholic acid, glycidodeoxycholic acid and spot 4 (unknown) were markers for distinguishing cattle bile and sheep bile. Conclusion The TLC analysis method could be a reference due to its simplicity, sensitivity and accuracy compared with traditional TLC method.

Key words: cattle bile, sheep bile, TLC, identification, chemometrics

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