多成分定性及定量结合化学计量学分析胀果甘草主要特征成分

doi:10.19803/j.1672-8629.2021.09.07

中国药物警戒 ›› 2021, Vol. 18 ›› Issue (9): 826-830.
DOI: 10.19803/j.1672-8629.2021.09.07

多成分定性及定量结合化学计量学分析胀果甘草主要特征成分

陈佳, 聂黎行^Δ, 程显隆, 胡晓茹, 魏锋^#, 马双成^*

中国食品药品检定研究院,北京 102629

收稿日期:2021-04-19 出版日期:2021-09-15 发布日期:2021-09-18
通讯作者: ^*马双成,男,博士,研究员,中药药效（毒效）物质基础及质量控制技术研究。E-mail: masc@nifdc.org.cn,^#为共同通信作者。
作者简介:陈佳,女,副研究员,中药质量评价。^Δ为并列第一作者。
基金资助:
“重大新药创制国家科技重大专项2018年度（2018ZX09735006）; 国家重点研发计划（2019YFC1711500）

Qualitative and Quantitative Analysis of Main Characteristic Components of Glycyrrhiza inflate Bat. with Chemometrics

CHEN Jia, NIE Lixing^Δ, CHENG Xianlong, HU Xiaoru, WEI Feng^#, MA Shuangcheng^*

National Institutes for Food and Drug Control, Beijing 102629, China

Received:2021-04-19 Online:2021-09-15 Published:2021-09-18

摘要/Abstract

摘要： 目的寻找胀果甘草区别于乌拉尔甘草的主要特征成分,为胀果甘草质量控制提供参考。方法采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS/MS）技术对胀果甘草及乌拉尔甘草化学成分进行检测,结合数据分析软件,以主成分分析（PCA）及正交偏最小二乘法分析（OPLS-DA）,对采集的数据进行分析,找出胀果甘草区别于乌拉尔甘草的差异性化合物。建立胀果甘草主要特征成分的高效液相色谱法（HPLC）含量测定方法,并对两者含量进行分析。结果确定了甘草查耳酮A、甘草查耳酮B、刺甘草查耳酮及光甘草酚是胀果甘草区别于乌拉尔甘草的主要特征成分,并建立了多指标同时测定的HPLC含量测定方法。结论建立的胀果甘草主要特征成分HPLC含量测定方法经方法学验证,可用于甘草查耳酮A、甘草查耳酮B、刺甘草查耳酮及光甘草酚的含量测定,为胀果甘草的质量控制提供科学依据。

关键词: 胀果甘草, 多成分定量, 特征成分, 超高效液相色谱-四极杆飞行时间质谱, 高效液相色谱, 化学计量学

Abstract: Objective To identify the main characteristic components of Glycyrrhiza inflate Bat. which distinguish this herbal medicine from Glycyrrhiza uralensis Fisch., and to provide reference for quality control of Glycyrrhiza inflate Bat. Methods Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) technology was used to detect the composition of Glycyrrhiza inflate Bat. and Glycyrrhiza uralensis Fisch. Combined with data analysis software, principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA) were conducted to analyze the collected data in order to reveal the difference in compounds between Glycyrrhiza inflate Bat. and Glycyrrhiza uralensis Fisch. A high performance liquid chromatography (HPLC) method was established for the determination of the main characteristic components of Glycyrrhiza inflate Bat. and Glycyrrhiza uralensis Fisch. Results Licochalcone A, licochalcone B, echinatin and glabrol were identified as the main characteristic components of Glycyrrhiza inflate Bat. that were different from those of Glycyrrhiza uralensis Fisch. An HPLC method for simultaneous determination of the above characteristic components was established. Conclusion This method has proved applicable to determination of licochalcone A, licochalcone B, echinatin and glabrol in Glycyrrhiza inflate Bat., thus contributing to quality control of Glycyrrhiza inflate Bat.

Key words: Glycyrrhiza inflate Bat., multi-component quantification, characteristic components, UPLC-Q-TOF-MS/MS, HPLC, chemometrics

中图分类号:

R927.1

陈佳, 聂黎行, 程显隆, 胡晓茹, 魏锋, 马双成. 多成分定性及定量结合化学计量学分析胀果甘草主要特征成分[J]. 中国药物警戒, 2021, 18(9): 826-830.

CHEN Jia, NIE Lixing, CHENG Xianlong, HU Xiaoru, WEI Feng, MA Shuangcheng. Qualitative and Quantitative Analysis of Main Characteristic Components of Glycyrrhiza inflate Bat. with Chemometrics[J]. Chinese Journal of Pharmacovigilance, 2021, 18(9): 826-830.

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多成分定性及定量结合化学计量学分析胀果甘草主要特征成分

Qualitative and Quantitative Analysis of Main Characteristic Components of Glycyrrhiza inflate Bat. with Chemometrics

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