基于植物代谢组学技术研究何首乌和制何首乌的差异性成分

doi:10.19803/j.1672-8629.2022.06.07

中国药物警戒 ›› 2022, Vol. 19 ›› Issue (6): 615-619.
DOI: 10.19803/j.1672-8629.2022.06.07

• 何首乌质量及安全性评价专栏（一） • 上一篇下一篇

基于植物代谢组学技术研究何首乌和制何首乌的差异性成分

杨建波, 汪祺^△, 王雪婷, 程显隆, 王莹, 高慧宇, 宋云飞, 魏锋^#, 马双成^*

中国食品药品检定研究院北京 100050

收稿日期:2022-02-17 出版日期:2022-06-15 发布日期:2022-06-13
通讯作者: ^* 马双成,男,博士,研究员,中药民族药质量标准及安全性评价研究。E-mail：masc@nifdc.org.cn;^#为共同通信作者。
作者简介:杨建波,男,博士,副研究员,常用中药活性、毒性成分及质量标准研究。^△为并列第一作者。
基金资助:
国家自然科学基金资助项目（81973476,81773874）

Difference analysis of chemical composition of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata based on plant metabonomics

YANG Jianbo, Wang Qi^△, WANG Xueting, CHENG Xianlong, WANG Ying, GAO Huiyu, SONG Yunfei, WEI Feng^#, MA Shuangcheng^*

National Institutes for Food and Drug Control, Beijing 100050, China

Received:2022-02-17 Online:2022-06-15 Published:2022-06-13

摘要/Abstract

摘要： 目的利用植物代谢组学技术对比分析何首乌（PMR）和制何首乌（PMRP）化学成分的差异,以阐明何首乌炮制前后化学成分的变化规律及炮制减毒的物质基础。方法采用超高效液相色谱-四级杆飞行时间质谱（UPLC-Q-TOF-MS/MS）技术对何首乌和制何首乌中成分进行检测;采用主成分分析（PCA）法观察样本的整体分布趋势;采用统计学等分析技术对火山图中差异性化合物进行筛选;并将所得差异性成分与Agilent PCDL自建数据库进行比对,对其化学成分进行初步鉴定。结果何首乌和制何首乌中的化学成分差异较大,从何首乌炮制后含量大幅减低的化合物中,初步鉴别45个化合物。结论何首乌炮制前后化学成分存在较大的差异,尤其蒽醌（单核和双核蒽醌）和二苯乙烯苷类成分存在明显的差异,这些差异性化合物可能是何首乌炮制减毒的主要物质基础。

关键词: 何首乌, 制何首乌, 代谢组学, 主成分分析, 火山图, 差异性化合物, 蒽醌类, 二苯乙烯苷类

Abstract: Objective To explore the chemical differences between Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Preparata (PMRP) with the help of plant metabonomics in order to clarify the regularity of chemical composition change before and after processing and the toxicity-attenuating material basis. Methods An ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) based metabolomics method was selected to analysis the composition of PMR and PMRP. Principal component analysis (PCA) method was used to observe the overall distribution trend of samples. Statistical analysis techniques were used to screen the differential compounds in the volcano plot. By comparing Agilent Natural Product Personal Compound Database and Library (PCDL), the chemical compositions were preliminarily identified. Results There were significant differences between PMR and PMRP. 45 differential compounds were tentatively identified and their contents were significantly decreased after processing. Conclusion There were significantly difference compounds between PMR and PMRP, especially for anthraquinone (mono and binuclear anthraquinone) and stilbene glycosides, which might be the main material basis for attenuating toxicity of Polygoni multiflorum Thunb.

Key words: Polygoni Multiflori Radix, Polygonum Multiflori Radix Preparata, metabonomics, principal component analysis, Volcano plot, difference compounds, anthrones, stilbene glycosides

中图分类号:

R917
R961

杨建波, 汪祺, 王雪婷, 程显隆, 王莹, 高慧宇, 宋云飞, 魏锋, 马双成. 基于植物代谢组学技术研究何首乌和制何首乌的差异性成分[J]. 中国药物警戒, 2022, 19(6): 615-619.

YANG Jianbo, Wang Qi, WANG Xueting, CHENG Xianlong, WANG Ying, GAO Huiyu, SONG Yunfei, WEI Feng, MA Shuangcheng. Difference analysis of chemical composition of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata based on plant metabonomics[J]. Chinese Journal of Pharmacovigilance, 2022, 19(6): 615-619.

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基于植物代谢组学技术研究何首乌和制何首乌的差异性成分

Difference analysis of chemical composition of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata based on plant metabonomics

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