基于超高效液相色谱-四极杆飞行时间质谱法分析乌拉尔甘草与光果甘草化学成分差异

doi:10.19803/j.1672-8629.2022.09.05

中国药物警戒 ›› 2022, Vol. 19 ›› Issue (9): 954-958.
DOI: 10.19803/j.1672-8629.2022.09.05

基于超高效液相色谱-四极杆飞行时间质谱法分析乌拉尔甘草与光果甘草化学成分差异

陈佳, 聂黎行^Δ, 胡晓茹, 刘薇, 魏锋^#, 马双成^*

中国食品药品检定研究院,北京 102629

收稿日期:2021-05-06 出版日期:2022-09-15 发布日期:2022-09-16
通讯作者: ^*马双成,男,博士,研究员,中药质量控制。E-mail: masc@nifdc.org.cn。^#为共同通信作者。
作者简介:陈佳,女,副研究员,中药质量评价。^Δ为并列第一作者。
基金资助:
重大新药创制国家科技重大专项2018年度（2018ZX09735006）; 国家重点研发计划（2019YFC1711500）

Differences of chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. by UHPLC-Q-TOF-MS/MS

CHEN Jia, NIE Lixing^Δ, HU Xiaoru, LIU Wei, WEI Feng^#, MA Shuangcheng^*

National Institutes for Food and Drug Control, Beijing 102629, China

Received:2021-05-06 Online:2022-09-15 Published:2022-09-16

摘要/Abstract

摘要： 目的识别豆科植物甘草（Glycyrrhiza uralensis Fisch.）（俗称“乌拉尔甘草”）与光果甘草（Glycyrrhiza glabra L.）的差异性化学成分,为甘草质量评价提供参考。方法采用超高效液相色谱-四极杆飞行时间质谱（UHPLC-Q-TOF-MS/MS）法对乌拉尔甘草及光果甘草化学成分进行检测,结合数据分析软件,以主成分分析（PCA）及正交偏最小二乘法分析（OPLS-DA）对采集的数据进行分析,找出乌拉尔甘草及光果甘草的差异性化合物。采用超高效液相色谱-三重四极杆质谱（UHPLC-TQ-MS/MS）法进一步验证差异性化合物的准确性。结果确定了光甘草定、新甘草苷、光甘草酚等11个化合物是乌拉尔甘草及光果甘草的差异性化学成分。结论乌拉尔甘草与光果甘草部分化学成分存在明显差异,这些差异性化学成分对甘草质量标准制定具有借鉴意义。

关键词: 甘草（乌拉尔甘草）, 光果甘草, 超高效液相色谱-四极杆飞行时间质谱, 超高效液相色谱-三重四极杆质谱, 主成分分析, 正交偏最小二乘法, 差异性化合物

Abstract: Objective To identify the differential chemical compounds of Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L., so as to provide reference for quality evaluation of licorice. Methods Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) technology was used to detect the composition of Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L.. Using data analysis software, principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA) were conducted to analyze the collected data in order to reveal the difference in compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L.. Ultra performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-TQ-MS/MS) was conducted to further verify the accuracy of detection of the differential compounds. Results Eleven compounds such as glabridin, neoliquiritin and glabrol were identified as the differential chemical components of Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L.. Conclusion There are obvious differences in some chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L., which can be used for reference in the formulation of quality standards for licorice.

Key words: Glycyrrhiza uralensis Fisch., Glycyrrhiza glabra L., UHPLC-Q-TOF-MS/MS, UHPLC-TQ-MS/MS, PCA, OPLS-DA, differential compounds

中图分类号:

R917

陈佳, 聂黎行, 胡晓茹, 刘薇, 魏锋, 马双成. 基于超高效液相色谱-四极杆飞行时间质谱法分析乌拉尔甘草与光果甘草化学成分差异[J]. 中国药物警戒, 2022, 19(9): 954-958.

CHEN Jia, NIE Lixing, HU Xiaoru, LIU Wei, WEI Feng, MA Shuangcheng. Differences of chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. by UHPLC-Q-TOF-MS/MS[J]. Chinese Journal of Pharmacovigilance, 2022, 19(9): 954-958.

参考文献

[1] Pharmacopoeia Committee of Ministry of Health of People’s Republic of China. Chinese Pharmacopoeia Part I (中华人民共和国药典一部)[M]. Beijing: China Medical Science Press, 2020: 88-89.
[2] DOU KN, SHANG XB, LIU SY, et al.Study on the effect of the licorice and active ingredients on the bread preservation[J]. Food Industry(食品工业), 2018, 39(3): 126-128.
[3] SUN J, ZHUANG YX, HU FQ, et al.Research progress of glycyrrhizin sweetener[J]. Chin Food Additives(中国食品添加剂), 2020, 11: 144-148.
[4] WANG JX.Introduction of natural ingredients in cosmetics[J]. Detergent & Cosmetics(日用化学品科学), 2019, 42(4): 43-47.
[5] CAO SY, SONG Z, LIN XY, et al.Comparative study on whitening activity of different solvent extracts from Glycyrrhiza[J]. J Changchun UnivTradit Chin Med(长春中医药大学学报), 2019, 35(1): 140-146.
[6] WANG JN, ZHOU YF, CUI YY, et al.Research progress on material basis and mechanism of Glycyrrhizae Radix et Rhizoma enhancing immunity[J]. Chin Tradit Herbal Drugs(中草药), 2021, 52(6): 1844-1850.
[7] DUAN HP, LIU HD, ZHAO YR, et al.Analysis of prescriptions of COVID-19 and plague related prescriptions in the Chinese medicine prescription dictionary[J]. Asia-pacific Tradit Med(亚太传统医药), 2021, 17(30): 153-158.
[8] XIANG C, QIAO X, YE M, et al.Classification and distribution analysis of components in Glycyrrhiza using licorice compounds database[J]. Acta Pharm Sin(药学学报), 2012, 47(8): 1023-1030.
[9] ZHANG QY, YE M.Chemical analysis of the Chinese herbal medicine Gan-Cao (licorice)[J]. J Chromatogr A, 2009, 1216(11):1954-69.
[10] CHEN J, WEI F, MA SC.Application of analytical chemistry in the quality evaluation of GlycyrrhizaSpp[J]. J LiqChromatogr RT, 2019, 42(5-6): 122-127.
[11] CHENG MZ, ZHANG JQ, YANG L, et al.Recent advances in chemical analysis of licorice (Gan-Cao)[J]. Fitoterapia, 2021,149(3): 104803.
[12] MOURBOUL A, MAO XM, RENA K, et al.Antioxidant activities of flavonoids from Xinjiang Glycyrrhiza glabra[J]. Chin Pharm J(中国药学杂志), 2008, 43(21): 1617-1620.
[13] XIE MC, DING JY, LI G.A comparative study on scavenging reactive oxygen species of GlycyrrhizaglabraangG. uralensis[J]. J Plant Resources and Environment(植物资源与环境学报), 2003, 12(1): 29-31.
[14] YANG FL, CHU TT, ZHANG YJ, et al.Quality assessment of licorice (Glycyrrhiza glabra L.) from different sources by multiple fingerprint profiles combined with quantitative analysis, antioxidant activity and chemometricmethods[J]. Food Chem, 2020, 15: 126854.
[15] GIOVANNI R, ELISA S, MARTA R, et al.A new exploration of licorice metabolome[J]. Food Chem, 2017, 15: 959-968.
[16] LUO ZL, LI Q, QIN JP, et al.Advances in studies on Glycyrrhiza glabra[J]. Chin Tradit Herbal Drugs(中草药), 2011, 42(10): 2154-2158.
[17] ZHU ZH, TAO WW, LI JP, et al.Rapid determination of flavonoids in licorice and comparison of three licorice species[J].Journal of Separation Science, 2016, 39, 473~482.
[18] CHARLOTTE S, TRISTESSE J, JEFFREY R A, et al.Species-specific Standardisation of Licorice by Metabolomic Profiling of Flavanones and Chalcones[J]. Phytochem Anal, 2014, 25(4): 378~388.
[19] LIN LC, RUAN JJ.HPLC determinated the content of Glabridin from Glycyrrhiza glabra L.[J]. Mod Chin Med(中国现代中药),2012, 14(6): 4-6.
[20] ZHU H, SHAO GQ, WANG WJ.Research progress on extraction of glabridin from Glycyrrhiza glabra L.[J].Chem Engin Euqipment(化学工程与装备), 2018, 9: 280-281.
[21] XIE LL, GAO ZK, ZHOU H, et al.Research progress on extraction, separation, detection and preparation of glabridin[J]. J China Pharm(中国药房), 2018, 29(21): 3009-3013.
[22] DU L, CHANG B, ZHANG Q, et al.Chemical constituents of flavonoids from roots of Glycyzzhiza glabra L.[J]. Chin Tradit Herbal Drugs(中草药), 2018, 49(20): 4780-4784.
[23] HU T, GAO ZQ, YIN YC, et al.Determination of seven flavonoids in Glycyrrhiza uralensis Fisch. and Glycyzzhiza glabra L. by UPLC[J]. Chin J Pharm Anal(药物分析杂志), 2019, 39(5): 763-771.

基于超高效液相色谱-四极杆飞行时间质谱法分析乌拉尔甘草与光果甘草化学成分差异

Differences of chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. by UHPLC-Q-TOF-MS/MS

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

[1]	王超, 王宇光, 马增春, 高月. 基于超高效液相色谱-四极杆飞行时间质谱法分析苦参藜芦配伍禁忌[J]. 中国药物警戒, 2023, 20(2): 163-167.
[2]	王亚丹, 闫建功, 陈明慧, 逄瑜, 戴忠, 马双成. 不同厂家雷公藤多苷片的超高效液相色谱指纹图谱研究[J]. 中国药物警戒, 2023, 20(2): 146-151.
[3]	杨建波, 汪祺, 王雪婷, 程显隆, 王莹, 高慧宇, 宋云飞, 魏锋, 马双成. 基于植物代谢组学技术研究何首乌和制何首乌的差异性成分[J]. 中国药物警戒, 2022, 19(6): 615-619.
[4]	陈佳, 聂黎行, 程显隆, 胡晓茹, 魏锋, 马双成. 多成分定性及定量结合化学计量学分析胀果甘草主要特征成分[J]. 中国药物警戒, 2021, 18(9): 826-830.
[5]	徐瑾,李馨龄,周鹃. 主成分聚类分析在药品不良反应报告综合质量评价中的应用[J]. 中国药物警戒, 2015, 12(2): 92-95.