中国药物警戒 ›› 2013, Vol. 10 ›› Issue (7): 390-392.

• 基础与临床研究 • 上一篇    下一篇

桦褐孔菌不同提取物小鼠急性毒性实验研究

刘向辉,谢人明,陈琳,李振志,孙文基,赵晔*   

  1. 西北大学陕西省生物医药重点实验室,陕西西安 710069
  • 收稿日期:2012-12-26 修回日期:2016-03-09 出版日期:2013-07-08 发布日期:2016-03-09
  • 通讯作者: 赵晔,博士,副教授,中药新药研究和开发。 E-mail: zhaoye@nwu.edu.cn
  • 作者简介:刘向辉,男,在读硕士,中药新药研究和开发。

Experimental Study on Mice's Acute Toxicity of Different Compositions in Inonotus Obliquus(Fr.) Pilat

LIU Xiang-hui, XIE Ren-ming, CHEN Lin, LI Zhen-zhi, SUN Wen-ji, ZHAO Ye   

  1. Biomedicine Key Laboratory of Shanxi Province, Northwest University, Shanxi Xi'an 710069, China
  • Received:2012-12-26 Revised:2016-03-09 Online:2013-07-08 Published:2016-03-09

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摘要： 目的 研究桦褐孔菌不同提取物对小鼠急性毒性作用,为临床安全用药提供依据。方法 采用经典的急性毒性实验方法,测定桦褐孔菌水提物和70%乙醇提取物的半数致死量（LD50）,并观察其毒性反应。结果 随着给药量的增大,桦褐孔菌水提物和醇提取物对小鼠毒性作用逐渐增强,死亡率逐渐升高,空白组没有异常变化。按生药量计算水提物的LD50 是17.49g·kg-1,95%可信区间为16.36～18.70g·kg-1；醇提取物LD50 是61.97g·kg-1,95%可信区间为53.73～71.46g·kg-1。结论 桦褐孔菌水提物和70%乙醇提取物均有一定的毒性,且水提物毒性起效量比70%乙醇提取物的起效量小。

关键词: 桦褐孔菌, 水提物, 醇提物, 急性毒性

Abstract: Objective The acute toxicity of different components in Inonotus obliquus(Fr.) Pilat on mice was studied in order to provide a basis for safe and reasonable medication in clinic. Methods Using classical Methods of acute toxicity tested, the median lethal dose(LD50) of water extraction components and 70% alcohol extraction components of Inonotus obliquus(Fr.) Pilaton mice were tested besides its toxic reaction. Results The higher the dose of water extraction and 70% ethanol extraction components increased, the more obvious the acute toxicity appeared to be, the more the mice died, and the control group showed normal. As to crude drug per kilogram body weight, the LD50 of water extraction components on mice was 17.49g·kg-1, moreover the 95% confidence limit was 16.36～18.70g·kg-1; the LD50 of 70% alcohol extraction components was 61.97g·kg -1, in addition the 95% confidence limit was 53.73～71.46g·kg -1. Conclusion The water extraction components and 70% alcohol extraction components of Inonotus obliquus(Fr.) Pilat had some toxicity, but the working quantity of water extraction components's toxic effect was lower than 70% alcohol extraction components.

Key words: Inonotus Obliquus(Fr.) Pilat , water extraction components, alcohol extraction components, acute toxicity

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