中国药物警戒 ›› 2013, Vol. 10 ›› Issue (10): 583-587.

• 附子急性毒性研究 • 上一篇    下一篇

附子不同组分对正常小鼠的急性毒性实验比较研究

李晓骄阳1,栾永福2,孙蓉3*   

  1. 1.中国药科大学,江苏南京211198；
    2.天津中医药大学,天津300073；
    3.山东省中医药研究院,山东济南250014
  • 收稿日期:2012-08-15 修回日期:2016-03-09 出版日期:2013-10-08 发布日期:2016-03-09
  • 通讯作者: 孙蓉,女,研究员·博导,博士,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:栾永福,男,在读硕士,中药药理学。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目课题2（2009CB522802）。

Comparative Study on Acute Toxicity of Different Components of on Normal Mice

Li Xiao-jiaoyang1 ,LUAN Yong-fu2 ,SUN Rong3*   

  1. 1.China Pharmaceutical University, Jiangsu Nanjing 211198, China;
    2.Tianjin University of Traditional Chinese Medicine Tianjin 300073, China;
    3.Shandong Research Academy of TCM, Shandong Jinan 250014, China
  • Received:2012-08-15 Revised:2016-03-09 Online:2013-10-08 Published:2016-03-09

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摘要： 目的 比较附子不同组分对正常小鼠急性毒性的影响,进行附子药材的急性毒性观察与评价,为临床安全合理用药提供依据。方法 分别制备附子全组分、水提组分和醇提组分,采用经典的急性毒性实验方法分别测定近似最小致死量（MLD）和半数致死量（LD50）。结果 附子醇提组分的对正常小鼠的LD50值及95%可信限为11.7g·kg-1·d-1 和11.1～12.4 g·kg-1·d-1,相当于临床70.0kg 的成人每公斤体重日用量的54.6 倍；附子全组分、水提组分对正常小鼠的MLD 为16.0 g·kg-1·d-1和14.4g·kg-1·d-1,相当于临床70.0kg 的成人每公斤体重日用量的74.7 倍和67.2 倍。通过观察发现,附子灌胃小鼠的急性毒性症状主要有:怠动、恶心、四肢麻痹、抽搐、惊厥和俯卧不动,且多发生于6.0h 之内。结论 附子不同组分对正常小鼠具有一定的急性毒性,其毒性强度大小为：醇提组分> 水提组分> 全组分。

关键词: 附子, 小鼠, 急性毒性

Abstract: Objective To compare the acute toxicity effect of different components of Aconite on normal mice and observe/evaluate the acute toxicity of Aconite herbs. Thus, we try to provide a foundation for rational drug use in clinic. Methods Preparing the all-components, water extracted components and alcohol extracted components, then ig. all-components, water extracted components and alcohol extracted components of Aconite to mice and the MLD, LD50 were tested respectively by the classic Methods of acute toxicity. Results The LD50 and 95% confidence limit of alcohol extracted components was 11.7 g·kg-1·d-1 and 11.1~12.4 g·kg-1·d-1 respectively, which was equal to 54.6 times of the clinical daily dosage in 70kg humans. The MLD of all-components was 16.0 g·kg-1·d-1 and the MLD of water extracted components was 14.4 g·kg-1·d-1, which was respectively equal to 74.7 times and 67.2 times of the clinical daily dosage in 70kg humans. By observing, we found that hypokinesia, nausea, quadriplegia, hyperspasmia, consequence and prostrate motionlessness were the main acute toxical symptoms and signs caused by Aconite, and these occurred in six hours after gavage administration. Conclusion Different components of Aconite can cause certain acute toxicity effect on normal mice, and the alcohol extracted components are with the greatest degree of toxicity, followed by water extracted components, and the least is the all-components.

Key words: mouse, acute toxicity

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