基于CiteSpace和生物信息学技术评估葛根素在肿瘤辅助治疗中的临床应用与安全风险

doi:10.19803/j.1672-8629.20230192

摘要/Abstract

摘要： 目的通过对葛根素辅助治疗肿瘤或癌症的文献进行可视化分析,得到葛根素在肿瘤或癌症上的临床应用范围及研究热点;进而通过生物信息学技术切入有效成分葛根素探讨其潜在的安全风险。方法通过系统整理有关葛根素预防癌症的有关资料,并利用CiteSpace软件,在文献数据库中检索自1990年1月1日至2022年10月31日有关葛根素预防肿瘤和癌症的有关资料进行了可视化分析,探索葛根素在癌症预防方面的医学应用意义;同时通过利用生物技术,对GEO数据库中的GSE85871基因芯片进行了检测和研究,对人乳腺癌细胞MCF7中的差异表达基因进行了GO功能注释研究和KEGG通路研究。结果基于可视化分析结果,葛根素在肝癌、肺癌、宫颈癌、膀胱癌、卵巢癌以及乳腺癌等病症的领域研究较多。但生物信息研究结果也表明,在应用葛根素辅助治疗乳腺癌中,葛根素会作用于cGMP信号通路、神经配体-受体相互作用信号通路,影响催产素分泌和破骨细胞分化,对心血管系统、神经系统和骨骼代谢可能存在潜在的安全风险。结论尽管葛根素在对肝癌、肺癌、宫颈癌等疾病的辅助治疗上有着潜在的使用意义,但在乳腺癌辅助治疗时则必须充分考虑其不良反应,应加强对含葛根素中药的合理应用研究,以确保其安全有效地使用。

关键词: 葛根素, 肿瘤, 肝癌, 肺癌, 乳腺癌, CiteSpace, 生物信息分析, 基因

Abstract: Objective To study the clinical applicability and hotspots of research of puerarin and to identify the potential risks to safety. Methods Based on exhaustive analysis of data on the anti-cancer properties of puerarin and using CiteSpace software, visual analysis was conducted by searching databases for the data on the prevention of tumors and cancer by puerarin that was published between January 1, 1990 and October 31, 2022. At the same time, the GSE85871 gene chip in GEO database was detected and studied using biotechnology while the GO function annotation and KEGG pathway of differentially expressed genes in human breast cancer cell MCF7 were explored. Results Visual analysis suggested that the role of puerarin in treating liver cancer, lung cancer, cervical cancer, bladder cancer, ovarian cancer, breast cancer and other diseases had been extensively studied. However, the results of bioinformatics research also showed that when puerarin was used in adjuvant treatment of breast cancer, puerarin tended to act on the cGMP signaling pathway and the nerve ligand receptor interaction signaling pathway, thus affecting oxytocin secretion and osteoclast differentiation. There might be potential safety risks to the cardiovascular system, nervous system and bone metabolism. Conclusion Despite the utility of puerarin in the adjuvant treatment of cancers, the related adverse reactions have to be taken into consideration, and rational use of traditional Chinese medicines containing puerarin should be recommended to ensure safe and effective use.

Key words: puerarin, tamor therapy, liver cancer, lang cancer, breast cancer, CiteSpace, bioinformatic analysis, gene

中图分类号:

刘亚迪, 王雪, 殷晓阳, 刘泽宇, 张晓朦, 张冰, 林志健. 基于CiteSpace和生物信息学技术评估葛根素在肿瘤辅助治疗中的临床应用与安全风险[J]. 中国药物警戒, 2023, 20(12): 1407-1414.

LIU Yadi, WANG Xue, YIN Xiaoyang, LIU Zeyu, ZHANG Xiaomeng, ZHANG Bing, LIN Zhijian. Clinical applicability and safety risks of puerarin in tumor adjuvant therapy based on CiteSpace and bioinformatics technology[J]. Chinese Journal of Pharmacovigilance, 2023, 20(12): 1407-1414.

参考文献

[1] SHI CX, DU JR, WU W, et al.Research progress on chemical components and pharmacological effects of Pueraria lobata[J]. Modern Chinese Medicine(中国现代中药), 2021, 23(12): 2177-2195.
[2] CAO P, ZHANG YS, WEI XM, et al.New progress in research on pharmacological effects of puerarin[J]. Traditional Chinese Patent Medicines and Simple Preparations(中成药), 2021, 43(8): 2130-2134.
[3] LIU XX, ZHAO W, WANG W, et al.Puerarin suppresses LPS-induced breast cancer cell migration, invasion and adhesion by blockage NF-κB and Erk pathway[J]. Biomedicine & Pharmacotherapy, 2017, 92: 429-436.
[4] LI XG.Effects of puerarin on proliferation and apoptosis of breast cancer cell line MCF-7[J]. Chinese Journal of Gerontology(中国老年学杂志), 2015, 35(15): 4167-4169.
[5] DING XL, HUANG ZK, AN XY, et al.Visualization analysis of traditional Chinese medicine induced cardiac adverse reactions based on CiteSpace knowledge graph[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2023, 20(2): 196-200.
[6] HUANG ZK, LIN ZJ, WANG Y, et al.Evaluation of the safety risk of emodin in the prevention and treatment of breast cancer based on bioinformatics technology[J]. Chinese Traditional Medicine Pharmacology and Clinic(中药药理与临床), 2020, 36(5): 126-130.
[7] YANG T, ZHANG XM, ZHANG B, et al.Research status of gouty arthritis at home and abroad: visualization analysis based on software[J]. Chinese Journal of Experimental Prescriptions(中国实验方剂学杂志), 2020, 26(20): 169-177.
[8] YANG XF, ZHU X, WANG MH, et al.Visual analysis of hyperuricemia and gout pathogenesis based on CiteSpace[J]. Chinese Medical Sciences(中国医药科学), 2022, 12(2): 46-49.
[9] ZOU XH, SUN Y.Bibliometrics analysis of the research status and trends of the association between depression and insulin from 2010 to 2020[J]. Frontiers in Psychiatry, 2021, 12: 683474-683474.
[10] YANG M, DING CB, MA JJ.Research progress on the pharmacological activity of puerarin[J]. Ginseng Research(人参研究), 2021, 33(6): 62-64.
[11] WANH WH, WANG GF, WANG L, et al.Study on the effect and mechanism of puerarin regulating signal on thyroid cancer cell apoptosis[J]. Traditional Chinese Medicine(中药材), 2018, 41(10): 2438-2441.
[12] LIU YL, WANG Y.Effect of puerarin on human pancreatic cancer cell proliferation and apoptosis[J]. Practical Pharmacy and Clinical Remedies(实用药物与临床), 2017, 20(11): 1244-1248.
[13] CHENG LQ.Puerarin induces apoptosis of cervical cancer cells through regulation related pathways[J]. Journal of Hubei University of Science and Technology Medical Edition(湖北科技学院学报医学版), 2020, 34(2): 96-98.
[14] LIN YJ, HOU YC, LIN CH, et al.Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells[J]. Biochemical and Biophysical Research Communications, 2009, 378(4): 683-688.
[15] LI ZF, XU WW, REN XY, et al.Puerarin promotes DUSP1 expression by regulating miR133a3p in breast cancer[J]. Molecular Medicine Reports, 2019, 19(1): 205-212.
[16] HE Q, CAO Y, CHEN JH.The effect of puerarin injection on hemorheology in patients with multiple myeloma[J]. China Science and Technology of Traditional Chinese Medicine(中国中医药科技), 2013, 20(2): 177-178.
[17] GUO W.Clinical observation and nursing of traditional Chinese and Western medicine in the treatment of endometrial and ovarian cancer[J]. Henan Traditional Chinese Medicine(河南中医), 2011, 31(6): 689-691.
[18] GU XX, LIU SL, TAO YH.Observation on the improvement of hypercoagulable state in 45 cases of malignant tumors with Shenmai and Puerarin injection[J]. Zhejiang Journal of Traditional Chinese Medicine(浙江中医杂志), 2005, 50(9): 413-413.
[19] XU B, LIANG RJ, LIU YX, et al.Downregulation - targeting α activation pathway promotes cell proliferation and malignant metastasis of breast cancer[J]. Progress in Modern General Surgery in China(中国现代普通外科进展), 2022, 25(9): 684-689.
[20] LIU X, WEI T, GAO ZD, et al.Kinases promote the migration of breast cancer cells by influencing calcium pool manipulating calcium channels[J]. Journal of Physiology(生理学报), 2019, 71(6): 874-882.
[22] LIN L, AI ZH.Promoting the proliferation and migration of ovarian cancer cells by regulating signaling pathways[J]. Modern Oncology Medicine(现在肿瘤医学), 2022, 30(22): 4027-4032.
[23] CHANG P, YU J, WANG JB, et al.The effect of aerobic exercise on myocardial injury and the cGMP PKG signaling pathway of natriuretic peptide in elderly mice[J]. Journal of Shanxi Medical University(山西医科大学学报), 2022, 53(11): 1418-1423.
[24] CHENG Y, YANG XY, ZHANG LB, et al.Mechanism study on the induction of renin secretion by cytomegalovirus infection[J]. Clinical Research(临床研究), 2020, 28(4): 33-35.
[25] PAN LZ, YAN ZY, ZUO CY, et al.The effect of long-term use of diazepam on the signaling pathway of neuroactive ligand receptor interaction[J]. Journal of China Pharmaceutical University(中国药科大学学报), 2011, 42(5): 443-446.
[26] LI YJ, YE BL, DU WJ, et al.Research progress on the neurobiological mechanism of oxytocin regulating anxiety and behavior[J]. Journal of Tongji University (Medical Edition)(同济大学学报医学版), 2022, 43(6): 889-894.
[27] CHEN F, REN GW, ZHANG XY, et al.Nuclear factors κ signal transduction mechanism of B receptor activator and activation of osteoclast[J]. China Tissue Engineering Research(中国组织工程研究), 2023, 27(2): 293-299.