中国药物警戒 ›› 2023, Vol. 20 ›› Issue (6): 671-674.
DOI: 10.19803/j.1672-8629.20220706

• 基础与临床研究 • 上一篇    下一篇

葛根参与淡豆豉发酵对体外抗骨质疏松活性的影响

张奥, 段天华, 徐荣荣, 姜晓娅, 张泽昭, 冯薇*, 牛丽颖#   

  1. 河北中医学院药学院,河北省中药配方颗粒技术创新中心,中药材品质评价与标准化河北省工程研究中心,河北 石家庄 050091
  • 收稿日期:2022-12-08 出版日期:2023-06-15 发布日期:2023-06-15
  • 通讯作者: * 冯薇,女,博士,教授,中药药效物质基础。E-mail: rainbow10571@126.com。#为共同通信作者。
  • 作者简介:张奥,男,在读硕士,中药药效物质基础。
  • 基金资助:
    国家自然科学基金资助项目(82104358); 河北省省级科技计划项目资助(21372503D, 22E50139D); 河北省自然科学基金面上项目资助(H2021423066)

Effect of involvement of Puerariae Lobatae Radix in fermentation of Semen Sojae Praeparatum on osteoporosis in vitro

ZHANG Ao, DUAN Tianhua, XU Rongrong, JIANG Xiaoya, ZHANG Zezhao, FENG Wei*, NIU Liying#   

  1. School of Pharmaceutical Sciences, Hebei TCM Formula Granule Technology Innovation Center, Hebei Engineering Research Center for Quality Evaluation & Standardization of Traditional Chinese Medicine, Shijiazhuang Hebei 050091, China
  • Received:2022-12-08 Online:2023-06-15 Published:2023-06-15

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摘要： 目的 增加葛根作为淡豆豉发酵辅料,探讨葛根参与淡豆豉发酵对体外抗骨质疏松活性的影响。方法 采用SPSS 22.0软件比较分析加葛根纯种菌发酵组、无葛根纯种菌发酵组、仅葛根辅料纯种菌发酵组、加葛根自然发酵组、无葛根自然发酵组和仅葛根辅料自然发酵组的药效活性,同时对比纯种发酵组1~7 d 的最优发酵时间。结果 抗骨质疏松作用最好的为加葛根纯种发酵4 d的淡豆豉,在1×10-4 g·L-1浓度下促成骨细胞增殖作用最佳,增殖率可达45.8%。结论 本研究证实葛根作为辅料发酵淡豆豉可有效提高体外抗骨质疏松活性,为传统中药发酵提供了新思路。

关键词: 淡豆豉, 发酵, 葛根, 体外活性, 成骨细胞, 骨质疏松, 增殖率

Abstract: Objective To investigate the effect of involvement of Puerariae Lobatae Radix in fermentation of Semen Sojae Praeparatum (SSP) on the anti-osteoporosis activity in vitro by adding Puerariae Lobatae Radix as an auxiliary material for fermentation of SSP. Methods The SPSS22.0 software was used to analyze the pharmacodynamic activities between the Puerariae Lobatae Radix-adding pure culture fermentation group, the pure culture fermentation without Puerariae Lobatae Radix group, the pure culture fermentation with Puerariae Lobatae Radix as the only auxiliary material group, the Pueraria lobata-involving natural fermentation group, the non-Pueraria lobata-involving natural fermentation group, and the Puerariae Lobatae Radix-involving alone natural fermentation group. At the same time, the optimum fermentation time within the first seven days was compared between pure culture fermentation groups. Results It was found that the best anti-osteoporosis condition was at the concentration of 1×10-4 g·L-1, SSP fermented with Pueraria for 4 days was the most effective in promoting osteoblast proliferation, and the proliferation rate was 45.8%. Conclusion This study has confirmed that Puerariae Lobatae Radix, when used as an auxiliary material for fermentation, can effectively improve the anti-osteoporosis activity in vitro, and provide new ideas for the fermentation of traditional Chinese medicines.

Key words: Semen Sojae Praeparatum, fermentation, Puerariae Lobatae Radix, in vitro activity, osteoblast, osteoporosis, proliferation rate

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