中国药物警戒 ›› 2022, Vol. 19 ›› Issue (10): 1078-1081.
DOI: 10.19803/j.1672-8629.20210196

• 基础与临床研究 • 上一篇    下一篇

齿瓣石斛多糖对D-半乳糖致氧化损伤小鼠的抗氧化作用

董寿堂, 张旭强, 王怀斌, 柴连周, 杨红芹, 胡燕   

  1. 保山中医药高等专科学校,云南 保山 678000
  • 收稿日期:2020-03-10 出版日期:2022-10-15 发布日期:2022-10-17
  • 作者简介:董寿堂,男,硕士,副教授,中草药现代药理学研究。
  • 基金资助:
    云南省教育厅科学研究基金项目（2019J0326）; 保山中医药高等专科学校科技计划重点项目（2019k009z）; 保山中医药高等专科学校科技创新团队项目(2020t001); 保山中医药高等专科学校百名中青年学术带头人资助项目（2020x002）

Antioxidant effect of Dendrobium devonianum polysaccharides on oxidative damage mice induced by D-galactose

DONG Shoutang, ZHANG Xuqiang, WANG huaibin, CHAI Lianzhou, YANG Hongqin, HU Yan   

  1. Baoshan College of Traditional Chinese Medicine, Baoshan Yunnan 678000, China
  • Received:2020-03-10 Online:2022-10-15 Published:2022-10-17

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摘要： 目的 研究齿瓣石斛多糖对D-半乳糖所致氧化损伤小鼠的抗氧化作用。方法 将72只雄性昆明种小鼠随机分成对照组,模型组,维生素C组及齿瓣石斛多糖高、中、低剂量组6组,每组12只,除对照组以外,其余各组均皮下注射120 mg·kg-1·d-1 D-半乳糖建立氧化损伤小鼠模型,同时对照组、模型组灌胃等量的生理盐水,维生素C组及齿瓣石斛多糖高、中、低剂量组分别以100、400、200、100 mg·kg-1·d-1灌胃给药,连续6周。于末次给药后1 h,测定小鼠血清、肝脏中丙二醛（malondialdehyde,MDA）、蛋白质羰基、还原型谷胱甘肽（glutathione,GSH）含量及超氧化物歧化酶（superoxide dismutase,SOD）活性。结果 与模型组比较,维生素C组及齿瓣石斛多糖高、中、低剂量组能明显提高小鼠血清中SOD 活性（P<0.05或P<0.01）;维生素C组及齿瓣石斛多糖高剂量组能明显提高小鼠肝脏中SOD 活性（P<0.05）,明显降低血清、肝脏中蛋白质羰基及MDA含量（P<0.05或P<0.01）,明显增加血清、肝脏中GSH含量（P<0.05）。结论 齿瓣石斛多糖具有一定的抗氧化作用。

关键词: 齿瓣石斛多糖, D-半乳糖, 小鼠, 抗氧化

Abstract: Objective To study the antioxidant effect of polysaccharides from Dendrobium devoninum on mice with oxidative damage induced by D-galactose. Methods Seventy-two mice were randomly divided into six groups: the control group, model group,vitamin C group, and high, medium, low dose groups of Dendrobium devonianum polysaccharides. Except the control group,mice in the other groups were injected with D-galactose 120 mg·kg-1·d-1 to establish a model of oxidative damage. For six weeks, the control group and model group were given normal saline of the equal volume by gavage, while the vitamin C group, high, medium and low dose groups of Dendrobium devonianum polysaccharides were treated with 100 mg·kg-1·d-1, 400 mg·kg-1·d-1, 200 mg·kg-1·d-1 and 100 mg·kg-1·d-1, respectively. One hour after the last administration,the activity of superoxide dismutase(SOD) and the contents of malondialdehyde (MDA),glutathione(GSH), and protein carbonyl in the serum and liver were detected. Results Compared with the model group, Dendrobium devonianum polysaccharides could significantly improve the activity of SOD and content of GSH, but decrease the contents of MDA and protein carbonyl in the serum and liver of mice with oxidative damage induced by D-galactose. Conclusion Dendrobium devonianum polysaccharides have antioxidant effect.

Key words: Dendrobium devonianum polysaccharides, D-galactose, mice, antioxidant

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