中国药物警戒 ›› 2021, Vol. 18 ›› Issue (4): 341-345.
DOI: 10.19803/j.1672-8629.2021.04.10

• 基础研究 • 上一篇    下一篇

糖尿病胃轻瘫动物模型建立方法初探

宋亚玲1, 黄举凯1, 丛佳林1, 程淑莉1, 张力1#, 杨晓晖2,*   

  1. 1北京中医药大学东方医院,北京 100078;
    2北京中医药大学东直门医院,北京 100700
  • 收稿日期:2020-07-06 出版日期:2021-04-15 发布日期:2021-04-23
  • 通讯作者: *杨晓晖,男,博士,主任医师,内分泌及代谢系统疾病的中医药治疗。E-mail: yxh0616@126.com ＃共同通信作者。
  • 作者简介:宋亚玲,女,在读博士,内分泌系统疾病的中医药临床与基础。
  • 基金资助:
    北京市科委“十病十药”研发专项课题（Z171100001717015）

Establishment of An Animal Model of Diabetic Gastroparesis

SONG Yaling1, HUANG Jukai1, CONG Jialin1, CHENG Shuli1, ZHANG Li1#, YANG Xiaohui2,*   

  1. 1*Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China;
    2*Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China
  • Received:2020-07-06 Online:2021-04-15 Published:2021-04-23

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摘要： 目的 探讨糖尿病胃轻瘫（diabetic gastroparesis, DGP）大鼠的造模方法。方法 从70只正常SD雄性大鼠中随机选择60只高脂高糖饲养2周后,禁食、自由饮水12 h,腹腔注射溶于柠檬酸盐缓冲液的链脲佐菌素（streptozotocin, STZ）50 mg/kg建立糖尿病大鼠模型。从50只糖尿病大鼠中随机选取10只高脂高糖饮食喂养8周,设为DGP模型组。余10只健康SD大鼠普通饲料饲养2周后腹腔注射等剂量柠檬酸盐缓冲液普通饲料喂养8周,设为对照组。检测造模期间大鼠血糖、体重变化及胃肠动力指标。结果 与正常组相比,模型组造模后空腹血糖显著增加（P<0.01）,8周后体重明显下降（P<0.01）,胃残留率增加（P<0.05）,小肠推进率显著降低（P<0.01）。结论 在腹腔注射STZ配合高脂高糖饲养诱导SD大鼠形成糖尿病基础上,高脂高糖喂养可构建DGP大鼠模型。

关键词: 糖尿病胃轻瘫, 动物模型, 链脲佐菌素, 高脂高糖饮食

Abstract: Objective To explore a method of establishing a rat model for diabetic gastroparesis (DGP). Methods Sixty rats were randomly chosen from seventy healthy SD male rats as a model group that was fed with a high-fat and high-sugar diet for two weeks. After being fasted and freely drinking water for 12h, these rats were injected intraperitoneally with streptozotocin (STZ soluble in 0.1mol/L citric acid buffered salt solution) 50mg/kg to establish a diabetic rat model. Fasting blood glucose≥13.9mmol/L after 72h indicated that a proper diabetic animal model was induced. From the fifty SD rats in the diabetic model, ten were randomly chosen to feed on a high-fat and high-sugar diet for eight weeks to establish a DGP animal model. Ten healthy SD male rats in the control group were fed for two weeks. After being fasted and freely drinking water for 12 h, they were injected intraperitoneally with an equivalent dosage of sodium citrate buffer and then fed with an ordinary diet for eight weeks as the normal control group. During the experiment, the general condition, body weight and blood glucose of rats in both groups were observed, while the gastric residual rate and small intestine advancing rate were measured after the rats were sacrificed. Results Compared with the normal group, the fasting blood glucose of the model group was significantly increased after modeling (P<0.01), and the rats became thinner, whose body weight and small bowel propulsion rate (P<0.01)were significantly reduced, while the gastric residue rate was significantly increased (P<0.01, P<0.05)after eight weeks. Conclusion Eight weeks of intraperitoneal injection of STZ 50mg/kg combined with a high-fat and high-sugar diet can induce a DGP rat model.

Key words: diabetic gastroparesis, animal model, Streptozotocin, high-fat and high-sugar diet

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